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验证数据展示

  • WB analysis of HeLa using 20483-1-AP (same clone as 20483-1-PBS)

    WB analysis of HeLa using 20483-1-AP (same clone as 20483-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20483-1-AP (WDR32 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

  • IP experiment of HepG2 using 20483-1-AP (same clone as 20483-1-PBS)

    IP experiment of HepG2 using 20483-1-AP (same clone as 20483-1-PBS)

    IP result of anti-WDR32 (IP:20483-1-AP, 4ug; Detection:20483-1-AP 1:300) with HepG2 cells lysate 6000ug. This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 20483-1-AP (same clone as 20483-1-PBS)

    IHC staining of human testis using 20483-1-AP (same clone as 20483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

  • IHC staining of human testis using 20483-1-AP (same clone as 20483-1-PBS)

    IHC staining of human testis using 20483-1-AP (same clone as 20483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 20483-1-AP (same clone as 20483-1-PBS)

    IF Staining of HepG2 using 20483-1-AP (same clone as 20483-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 20483-1-AP (WDR32 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20483-1-PBS in a different storage buffer formulation.

WDR32 Polyclonal antibody, PBS Only

WDR32 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20483-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

20483-1-PBS targets WDR32 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14360

Product name: Recombinant human WDR32 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-225 aa of BC003520

Sequence: TRFLMRMRLTPDCSKMLISTSSGYLLILHDLDLTNSLEVGSYPILRARRTTSSSGVSPRNSLEVVTPEVLGESDHGNCITSLQLHPKGWATLLRCSSNSDDEECTCVYEFQEGAPVRPVSPRCSLRLTHYIEEANVGRGYIKELCFSPDGRMISSPHGYGIRLLGFDKQCSELVDCLPKEASPLRVIRSLYSHNDVVLTTKFSPTHCQIASGCLSGRVSLYQPKF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 32
别名
计算分子量 559 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC003520
基因名称 WDR32
Gene ID (NCBI) 79269
RRIDAB_10695183
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5QP82
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WDR32, containing seven WD repeats, is also termed DDB1- and CUL4-associated factor 10 (DCAF10), which may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex (PMID: 16949367). WDR32 is able to interact with DDB1 (PMID: 16949367). WDR32 is supposed to be implicated in process of ubiquitin-dependent protein degradation system. Two isoforms are described and produced by alternative splicing. Proteintech's WDR32 antibody 20483-1-AP majorly detects WDR32 isoform1 with a theoretical MW of 61 kDa.

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