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验证数据展示

  • WB analysis of HeLa using 20483-1-AP

    WB analysis of HeLa using 20483-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20483-1-AP (WDR32 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 20483-1-AP

    IP experiment of HepG2 using 20483-1-AP

    IP result of anti-WDR32 (IP:20483-1-AP, 4ug; Detection:20483-1-AP 1:300) with HepG2 cells lysate 6000ug.

  • IHC staining of human testis using 20483-1-AP

    IHC staining of human testis using 20483-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 20483-1-AP

    IHC staining of human testis using 20483-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20483-1-AP (WDR32 antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HepG2 using 20483-1-AP

    IF Staining of HepG2 using 20483-1-AP

    Immunofluorescent analysis of HepG2 cells using 20483-1-AP (WDR32 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

WDR32 Polyclonal antibody

WDR32 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20483-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, ELISA

别名

DCAF10, WD repeat domain 32, WDR32

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells
Positive IP detected inHepG2 cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20483-1-AP targets WDR32 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14360

Product name: Recombinant human WDR32 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-225 aa of BC003520

Sequence: TRFLMRMRLTPDCSKMLISTSSGYLLILHDLDLTNSLEVGSYPILRARRTTSSSGVSPRNSLEVVTPEVLGESDHGNCITSLQLHPKGWATLLRCSSNSDDEECTCVYEFQEGAPVRPVSPRCSLRLTHYIEEANVGRGYIKELCFSPDGRMISSPHGYGIRLLGFDKQCSELVDCLPKEASPLRVIRSLYSHNDVVLTTKFSPTHCQIASGCLSGRVSLYQPKF

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 32
别名 DCAF10, WD repeat domain 32, WDR32
计算分子量 559 aa, 61 kDa
观测分子量 61 kDa
GenBank蛋白编号BC003520
基因名称 WDR32
Gene ID (NCBI) 79269
RRIDAB_10695183
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ5QP82
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR32, containing seven WD repeats, is also termed DDB1- and CUL4-associated factor 10 (DCAF10), which may function as a substrate receptor for CUL4-DDB1 E3 ubiquitin-protein ligase complex (PMID: 16949367). WDR32 is able to interact with DDB1 (PMID: 16949367). WDR32 is supposed to be implicated in process of ubiquitin-dependent protein degradation system. Two isoforms are described and produced by alternative splicing. Proteintech's WDR32 antibody 20483-1-AP majorly detects WDR32 isoform1 with a theoretical MW of 61 kDa.

实验方案

Product Specific Protocols
IF protocol for WDR32 antibody 20483-1-APDownload protocol
IHC protocol for WDR32 antibody 20483-1-APDownload protocol
IP protocol for WDR32 antibody 20483-1-APDownload protocol
WB protocol for WDR32 antibody 20483-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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