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验证数据展示

  • IHC staining of human testis using 12644-1-AP

    IHC staining of human testis using 12644-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human testis using 12644-1-AP

    IHC staining of human testis using 12644-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12644-1-AP (WDR16 Antibody) at dilution of 1:50 (under 40x lens).

  • IF Staining of HeLa using 12644-1-AP

    IF Staining of HeLa using 12644-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 12644-1-AP (WDR16 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

WDR16 Polyclonal antibody

WDR16 Polyclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 12644-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

WDRPUH, WD40-repeat protein up-regulated in HCC, WD repeat-containing protein 16, WD repeat domain 16, Cilia- and flagella-associated protein 52

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman testis tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12644-1-AP targets WDR16 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3281

Product name: Recombinant human WDR16 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 300-620 aa of BC025392

Sequence: GEGHQFLVGTEESHIYRVSFTDFKETLIATCHFDAVKDIVFPFGTAELFATCAKKDIRVWHTSSNRELLRITVPNMTCHGIDFMRDGKSIISAWNDGKIRAFAPETGRLMYVINNAHRIGVTAIATTSDCKRVISGGGEGEVRVWQIGCQTQKLEEALKEHKSSVSCIRVKRNNEECVTASTDGTCIIWDLVRLRRNQMILANTLFQCVCYHPEEFQIITSGTDRKIAYWEVFDGTVIRELEGSLSGSINGMDITQEGVHFVTGGNDHLVKVWDYNEGEVTHVGVGHSGNITRIRISPGNQYIVSVSADGAILRWKYPYTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 16
别名 WDRPUH, WD40-repeat protein up-regulated in HCC, WD repeat-containing protein 16, WD repeat domain 16, Cilia- and flagella-associated protein 52
计算分子量 620 aa, 68 kDa
GenBank蛋白编号BC025392
基因名称 WDR16
Gene ID (NCBI) 146845
RRIDAB_2272921
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8N1V2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for WDR16 antibody 12644-1-APDownload protocol
IHC protocol for WDR16 antibody 12644-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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