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验证数据展示

  • WB analysis using 17197-1-AP (same clone as 17197-1-PBS)

    WB analysis using 17197-1-AP (same clone as 17197-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17197-1-AP (WDFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17197-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 17197-1-AP (same clone as 17197-1-PBS)

    IF Staining of HeLa using 17197-1-AP (same clone as 17197-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WDFY2 antibody (17197-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 17197-1-PBS in a different storage buffer formulation.

WDFY2 Polyclonal antibody, PBS Only

WDFY2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 17197-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA

别名

Prof, Propeller-FYVE protein, WD repeat and FYVE domain-containing protein 2, WD40- and FYVE domain-containing protein 2, WDF2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17197-1-PBS targets WDFY2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag10415

Product name: Recombinant human WDFY2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 72-400 aa of BC014004

Sequence: SCMSFNPETRRLSIGLDNGTISEFILSEDYNKMTPVKNYQAHQSRVTMILFVLELEWVLSTGQDKQFAWHCSESGQRLGGYRTSAVASGLQFDVETRHVFIGDHSGQVTILKLEQENCTLVTTFRGHTGGVTALCWDPVQRVLFSGSSDHSVIMWDIGGRKGTAIELQGHNDRVQALSYAQHTRQLISCGGDGGIVVWNMDVERQETPEWLDSDSCQKCDQPFFWNFKQMWDSKKIGLRQHHCRKCGKAVCGKCSSKRSSIPLMGFEFEVRVCDSCHEAITDEERAPTATFHDSKHNIVHVHFDATRGWLLTSGTDKVIKLWDMTPVVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat and FYVE domain containing 2
别名 Prof, Propeller-FYVE protein, WD repeat and FYVE domain-containing protein 2, WD40- and FYVE domain-containing protein 2, WDF2
计算分子量 400 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC014004
基因名称 WDFY2
Gene ID (NCBI) 115825
RRIDAB_3741909
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96P53
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WDFY2, also named Prof, is a protein that contains WD repeats and an FYVE domain. WDFY2 regulates the membrane-bound matrix metalloproteinase MT1-MMP (MMP14) efflux by controlling endosome sorting. WDFY2 may act as a tumor suppressor by limiting VAMP3-dependent recycling to inhibit matrix metalloproteinase secretion and cell invasion (PMID: 31253801). WDFY2 in the cell nucleus directly participates in homologous recombination-mediated DNA repair (PMID: 41196680).

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