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Species Reactivity
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Conjugate
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Conjugate
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Compatible Species
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验证数据展示

  • WB analysis using 17197-1-AP

    WB analysis using 17197-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17197-1-AP (WDFY2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

WDFY2 Polyclonal antibody

WDFY2 Polyclonal Antibody for WB, ELISA
Cat No. 17197-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, ELISA

别名

ZFYVE22, WDF2, WD40- and FYVE domain-containing protein 2, WD repeat and FYVE domain-containing protein 2, Propeller-FYVE protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL-929 cells, 3T3-L1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17197-1-AP targets WDFY2 in WB, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse
免疫原 WDFY2 fusion protein Ag10415 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat and FYVE domain containing 2
别名 ZFYVE22, WDF2, WD40- and FYVE domain-containing protein 2, WD repeat and FYVE domain-containing protein 2, Propeller-FYVE protein
计算分子量 400 aa, 45 kDa
观测分子量45 kDa
GenBank蛋白编号BC014004
基因名称 WDFY2
Gene ID (NCBI) 115825
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96P53
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDFY2, also named Prof, is a protein that contains WD repeats and an FYVE domain. WDFY2 regulates the membrane-bound matrix metalloproteinase MT1-MMP (MMP14) efflux by controlling endosome sorting. WDFY2 may act as a tumor suppressor by limiting VAMP3-dependent recycling to inhibit matrix metalloproteinase secretion and cell invasion. (PMID: 31253801)

实验方案

Product Specific Protocols
WB protocol for WDFY2 antibody 17197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols