验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
28192-1-AP targets WBSCR22 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag28094 Product name: Recombinant human WBSCR22 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-142 aa of BC000169 Sequence: MASRGRRPEHGGPPELFYDETEARKYVRNSRMIDIQTRMAGRALELLYLPENKPCYLLDIGCGTGLSGSYLSDEGHYWVGLDISPAMLDEAVDREIEGDLLLGDMGQGIPFKPGTFDGCISISAVQWLCNANKKSENPAKRL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Williams Beuren syndrome chromosome region 22 |
| 别名 | Bud site selection protein 23 homolog, BUD23, HASJ4442, HUSSY 3, MERM1, Metastasis-related methyltransferase 1, PP3381, Probable 18S rRNA (guanine-N(7))-methyltransferase, rRNA methyltransferase and ribosome maturation factor, WBMT, WBSCR22 |
| 计算分子量 | 32 kDa |
| 观测分子量 | 32 kDa |
| GenBank蛋白编号 | BC000169 |
| 基因名称 | WBSCR22 |
| Gene ID (NCBI) | 114049 |
| RRID | AB_2918150 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O43709 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WBSCR22 (Williams-Beuren syndrome chromosomal region 22 protein), also known as BUD23, MERM1, is affected in Williams-Beuren syndrome and has been implicated in tumorigenesis and metastasis formation. WBSCR22 contains a nuclear localization signal and a common S-adenosyl-L-methionine binding motif that is evolutionarily conserved in methyltransferases (PMID: 29133897, 25525153). WBSCR22 protein is expressed at a high level in invasive breast cancer and its ectopic expression enhances tumor cell survival in the vasculature (PMID: 24086612).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for WBSCR22 antibody 28192-1-AP | Download protocol |
| WB protocol for WBSCR22 antibody 28192-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Heliyon RNA-related DNA damage and repair: The role of N7-methylguanosine in the cell nucleus exposed to UV light | ||
J Transl Med Histidine metabolism drives liver cancer progression via immune microenvironment modulation through metabolic reprogramming
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