验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, NIH/3T3 cells, MCF-7 cells, Jurkat cells |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| IF | See 1 publications below |
产品信息
66585-1-Ig targets WBP2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | Human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag2661 Product name: Recombinant human WBP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-261 aa of BC010616 Sequence: MALNKNHSEGGGVIVNNTESILMSYDHVELTFNDMKNVPEAFKGTKKGTVYLTPYRVIFLSKGKDAMQSFMMPFYLMKDCEIKQPVFGANYIKGTVKAEAGGGWEGSASYKLTFTAGGAIEFGQRMLQVASQASRGEVPSGAYGYSYMPSGAYVYPPPVANGMYPCPPGYPYPPPPPEFYPGPPMMDGAMGYVQPPPPPYPGPMEPPVSGPDVPSTPAAEAKAAEAAASAYYNPGNPHNVYMPTSQPPPPPYYPPEDKKTQ 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | WW domain binding protein 2 |
| 别名 | WBP 2, WBP2, WW domain binding protein 2 |
| 计算分子量 | 261 aa, 28 kDa |
| 观测分子量 | 30-40 kDa |
| GenBank蛋白编号 | BC010616 |
| 基因名称 | WBP2 |
| Gene ID (NCBI) | 23558 |
| RRID | AB_2881945 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q969T9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for WBP2 antibody 66585-1-Ig | Download protocol |
| IHC protocol for WBP2 antibody 66585-1-Ig | Download protocol |
| WB protocol for WBP2 antibody 66585-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
