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  • Featured Product
  • KD/KO Validated

WBP1 Polyclonal antibody

WBP1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 11042-1-AP

Print datasheet

Synonyms

WBP 1, WBP1, WW domain binding protein 1

验证数据展示

  • WB analysis of mouse brain using 11042-1-AP

    WB analysis of mouse brain using 11042-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 11042-1-AP (WBP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of human pancreas cancer using 11042-1-AP

    IHC staining of human pancreas cancer using 11042-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11042-1-AP (WBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11042-1-AP targets WBP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivitymouse
Immunogen WBP1 fusion protein Ag1478 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WW domain binding protein 1
Synonyms WBP 1, WBP1, WW domain binding protein 1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 32 kDa
GenBank Accession NumberBC010012
Gene Symbol WBP1
Gene ID (NCBI) 23559
RRIDAB_2092061
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96G27
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WBP1 was originally identified in vitro as a protein that bound to the WW domain of Yes kinase-associated protein. The silencing of WBP1 with small hairpin RNAs caused partial, but significant suppression of ATF6-induced apoptosis. It is a type I transmembrane protein.

实验方案

Product Specific Protocols
WB protocol for WBP1 antibody 11042-1-APDownload protocol
IHC protocol for WBP1 antibody 11042-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

J Biol Chem

Activating transcription factor-6 (ATF6) mediates apoptosis with reduction of myeloid cell leukemia sequence 1 (Mcl-1) protein via induction of WW domain binding protein 1.

Authors - Morishima Nobuhiro N
  • KD Validated
View Article