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验证数据展示

  • WB analysis using 86930-1-RR

    WB analysis using 86930-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86930-1-RR (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 86930-1-RR

    IHC staining of mouse heart using 86930-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86930-1-RR (Vinculin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Vinculin antibody included
  • IF Staining of mouse colon using 86930-1-RR

    IF Staining of mouse colon using 86930-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 86930-1-RR

    IF Staining of A549 using 86930-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 86930-1-RR

    Affinity and Kinetic Characterization of 86930-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86930-1-RR against Human Vinculin were performed. The affinity constant is below 1 pM.

Vinculin Recombinant monoclonal antibody

Vinculin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 86930-1-RR

产品说明书

CloneNo. 251919D10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, ELISA

别名

CMD1W, Metavinculin, MV, MVCL, VCL

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, L02 cells, HUVEC cells, HGC-27 cells, NIH/3T3 cells, HSC-T6 cells
Positive IHC detected inmouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue
Positive IF/ICC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy® Vinculin Ready-To-Use IHC Kit. Vinculin primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

86930-1-RR targets Vinculin in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag24942

Product name: Recombinant human Vinculin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 777-1066 aa of BC039174

Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vinculin
别名 CMD1W, Metavinculin, MV, MVCL, VCL
计算分子量 1133 aa, 124 kDa
观测分子量124 kDa
GenBank蛋白编号BC039174
基因名称 Vinculin
Gene ID (NCBI) 7414
RRIDAB_3745213
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18206
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein that is involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is a muscle-specific protein.

实验方案

Product Specific Protocols
IF protocol for Vinculin antibody 86930-1-RRDownload protocol
IHC protocol for Vinculin antibody 86930-1-RRDownload protocol
WB protocol for Vinculin antibody 86930-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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