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验证数据展示

  • WB analysis using 86930-1-RR (same clone as 86930-1-PBS)

    WB analysis using 86930-1-RR (same clone as 86930-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86930-1-RR (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 86930-1-RR (same clone as 86930-1-PBS)

    IHC staining of mouse heart using 86930-1-RR (same clone as 86930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86930-1-RR (Vinculin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Vinculin antibody included
  • IF Staining of A549 using 86930-1-RR (same clone as 86930-1-PBS)

    IF Staining of A549 using 86930-1-RR (same clone as 86930-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86930-1-RR

    Affinity and Kinetic Characterization of 86930-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86930-1-RR against Human Vinculin were performed. The affinity constant is below 1 pM.

Vinculin Recombinant monoclonal antibody, PBS Only

Vinculin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 86930-1-PBS

产品说明书

CloneNo. 251919D10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

CMD1W, Metavinculin, MV, MVCL, VCL

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® Vinculin Ready-To-Use IHC Kit. Vinculin primary antibody included.

产品信息

86930-1-PBS targets Vinculin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag24942

Product name: Recombinant human Vinculin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 777-1066 aa of BC039174

Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vinculin
别名 CMD1W, Metavinculin, MV, MVCL, VCL
计算分子量 1133 aa, 124 kDa
观测分子量124 kDa
GenBank蛋白编号BC039174
基因名称 Vinculin
Gene ID (NCBI) 7414
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18206
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein that is involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is a muscle-specific protein.

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