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验证数据展示

  • WB analysis using 86930-1-RR (same clone as 86930-1-PBS)

    WB analysis using 86930-1-RR (same clone as 86930-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86930-1-RR (Vinculin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 86930-1-RR (same clone as 86930-1-PBS)

    IHC staining of mouse heart using 86930-1-RR (same clone as 86930-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 86930-1-RR (Vinculin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Vinculin antibody included
  • IF Staining of mouse colon using 86930-1-RR (same clone as 86930-1-PBS)

    IF Staining of mouse colon using 86930-1-RR (same clone as 86930-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 86930-1-RR (same clone as 86930-1-PBS)

    IF Staining of A549 using 86930-1-RR (same clone as 86930-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Vinculin antibody (86930-1-RR, Clone: 251919D10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86930-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86930-1-RR

    Affinity and Kinetic Characterization of 86930-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86930-1-RR against Human Vinculin were performed. The affinity constant is below 1 pM.

Vinculin Recombinant monoclonal antibody, PBS Only

Vinculin Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 86930-1-PBS

产品说明书

CloneNo. 251919D10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

CMD1W, Metavinculin, MV, MVCL, VCL

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy® Vinculin Ready-To-Use IHC Kit. Vinculin primary antibody included.

产品信息

86930-1-PBS targets Vinculin in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag24942

Product name: Recombinant human Vinculin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 777-1066 aa of BC039174

Sequence: PKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vinculin
别名 CMD1W, Metavinculin, MV, MVCL, VCL
计算分子量 1133 aa, 124 kDa
观测分子量124 kDa
GenBank蛋白编号BC039174
基因名称 Vinculin
Gene ID (NCBI) 7414
RRIDAB_3745213
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP18206
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein that is involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein that is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is a muscle-specific protein.

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