WB result of Vimentin antibody (80232-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Vimentin transfected HeLa cells. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human melanoma slide using 80232-1-RR (Vimentin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​ The staining was performed on an autostainer. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human melanoma tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​ The staining was performed on an autostainer. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). The staining was performed on an autostainer. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2000. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

1x10^6 HeLa cells were intracellularly stained with 0.25 ug Vimentin Recombinant monoclonal antibody (80232-1-RR, Clone:6K21) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.