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验证数据展示

  • WB analysis using 80232-1-RR (same clone as 80232-1-PBS)

    WB analysis using 80232-1-RR (same clone as 80232-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80232-1-RR (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 80232-1-RR (same clone as 80232-1-PBS)

    WB analysis of A549 using 80232-1-RR (same clone as 80232-1-PBS)

    WB result of Vimentin antibody (80232-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Vimentin transfected HeLa cells. This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • IHC staining of human melanoma tissue using 80232-1-RR (same clone as 80232-1-PBS)

    IHC staining of human melanoma tissue using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human melanoma slide using 80232-1-RR (Vimentin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​ This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 80232-1-RR (same clone as 80232-1-PBS)

    IHC staining of human breast cancer using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:2500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Vimentin antibody included
  • IHC staining of human melanoma tissue using 80232-1-RR (same clone as 80232-1-PBS)

    IHC staining of human melanoma tissue using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human melanoma tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Staining was performed on the Epredia 480S Autostainer.​ This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 80232-1-RR (same clone as 80232-1-PBS)

    IHC staining of human ovary cancer using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Vimentin antibody included
  • IHC staining of human ovary cancer using 80232-1-RR (same clone as 80232-1-PBS)

    IHC staining of human ovary cancer using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Vimentin antibody included
  • IHC staining of human colon using 80232-1-RR (same clone as 80232-1-PBS)

    IHC staining of human colon using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Vimentin antibody included
  • IHC staining of human colon using 80232-1-RR (same clone as 80232-1-PBS)

    IHC staining of human colon using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Vimentin antibody included
  • IHC staining of human colon cancer using 80232-1-RR (same clone as 80232-1-PBS)

    IHC staining of human colon cancer using 80232-1-RR (same clone as 80232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80232-1-RR (Vimentin antibody) at dilution of 1:10000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Vimentin antibody included
  • IF Staining of mouse breast cancer using 80232-1-RR (same clone as 80232-1-PBS)

    IF Staining of mouse breast cancer using 80232-1-RR (same clone as 80232-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse breast cancer using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 80232-1-RR (same clone as 80232-1-PBS)

    IF Staining of HeLa using 80232-1-RR (same clone as 80232-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Vimentin antibody (80232-1-RR, Clone: 6K21 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 80232-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Vimentin Recombinant monoclonal antibody, PBS Only

Vimentin Uni-rAb® Recombinant Antibody for WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, Indirect ELISA
Cat No. 80232-1-PBS

产品说明书

CloneNo. 6K21

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, Indirect ELISA

别名

VIM, 6K21

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Vimentin Ready-To-Use IHC Kit. Vimentin primary antibody included.

产品信息

80232-1-PBS targets Vimentin in WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IHC-Autostainer, IF/ICC, IF-Fro, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag0489

Product name: Recombinant human Vimentin protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-466 aa of BC000163

Sequence: MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKGQGKSRLGDLYEEEMRELRRQVDQLTNDKARVEVERDNLAEDIMRLREKLQEEMLQREEAENTLQSFRQDVDNASLARLDLERKVESLQEEIAFLKKLHEEEIQELQAQIQEQHVQIDVDVSKPDLTAALRDVRQQYESVAAKNLQEAEEWYKSKFADLSEAANRNNDALRQAKQESTEYRRQVQSLTCEVDALKGTNESLERQMREMEENFAVEAANYQDTIGRLQDEIQNMKEEMARHLREYQDLLNVKMALDIEIATYRKLLEGEESRISLPLPNFSSLNLRETNLDSLPLVDTHSKRTLLIKTVETRDGQVINETSQHHDDLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vimentin
别名 VIM, 6K21
计算分子量 466 aa, 54 kDa
观测分子量50-55 kDa
GenBank蛋白编号BC000163
基因名称 VIM
Gene ID (NCBI) 7431
ENSEMBL Gene IDENSG00000026025
RRIDAB_2918879
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP08670
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).