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验证数据展示

  • WB analysis using 84472-4-RR

    WB analysis using 84472-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84472-4-RR (VPS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 84472-4-RR

    WB analysis of HeLa using 84472-4-RR

    WB result of VPS28 antibody (84472-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS28 transfected HeLa cells.

  • FC experiment of SH-SY5Y using 84472-4-RR

    FC experiment of SH-SY5Y using 84472-4-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug VPS28 Recombinant antibody (84472-4-RR, Clone:241886D3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of A549 using 84472-4-RR

    IF Staining of A549 using 84472-4-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using VPS28 antibody (84472-4-RR, Clone: 241886D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84472-4-RR

    Affinity and Kinetic Characterization of 84472-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84472-4-RR against Human VPS28 were performed. The affinity constant is 1.72 nM.

  • Featured Product
  • KD/KO验证

VPS28 Recombinant monoclonal antibody

VPS28 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 84472-4-RR

产品说明书

CloneNo. 241886D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

241886D3, ESCRT-I complex subunit VPS28, H-Vps28, Vacuolar protein sorting-associated protein 28 homolog

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Jurkat cells, THP-1 cells, U-251 cells
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inSH-SY5Y cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84472-4-RR targets VPS28 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7784

Product name: Recombinant human VPS28 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-221 aa of BC006485

Sequence: MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACSRLLVQYKAAFRQVQGSEISSIDEFCRKFRLDCPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRFLHA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vacuolar protein sorting 28 homolog (S. cerevisiae)
别名 241886D3, ESCRT-I complex subunit VPS28, H-Vps28, Vacuolar protein sorting-associated protein 28 homolog
计算分子量 25 kDa
观测分子量 28 kDa
GenBank蛋白编号BC006485
基因名称 VPS28
Gene ID (NCBI) 51160
RRIDAB_3671995
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UK41
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.

实验方案

Product Specific Protocols
FC protocol for VPS28 antibody 84472-4-RRDownload protocol
IF protocol for VPS28 antibody 84472-4-RRDownload protocol
WB protocol for VPS28 antibody 84472-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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