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验证数据展示

  • WB analysis using 84472-4-RR (same clone as 84472-4-PBS)

    WB analysis using 84472-4-RR (same clone as 84472-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84472-4-RR (VPS28 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84472-4-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 84472-4-RR (same clone as 84472-4-PBS)

    WB analysis of HeLa using 84472-4-RR (same clone as 84472-4-PBS)

    WB result of VPS28 antibody (84472-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VPS28 transfected HeLa cells. This data was developed using the same antibody clone with 84472-4-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 84472-4-RR (same clone as 84472-4-PBS)

    FC experiment of SH-SY5Y using 84472-4-RR (same clone as 84472-4-PBS)

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug VPS28 Recombinant antibody (84472-4-RR, Clone:241886D3) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84472-4-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 84472-4-RR (same clone as 84472-4-PBS)

    IF Staining of A549 using 84472-4-RR (same clone as 84472-4-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using VPS28 antibody (84472-4-RR, Clone: 241886D3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84472-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84472-4-RR

    Affinity and Kinetic Characterization of 84472-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84472-4-RR against Human VPS28 were performed. The affinity constant is 1.72 nM.

  • Featured Product
  • KD/KO验证

VPS28 Recombinant monoclonal antibody, PBS Only

VPS28 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84472-4-PBS

产品说明书

CloneNo. 241886D3

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), Indirect ELISA

别名

241886D3, ESCRT-I complex subunit VPS28, H-Vps28, Vacuolar protein sorting-associated protein 28 homolog

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

84472-4-PBS targets VPS28 in WB, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag7784

Product name: Recombinant human VPS28 protein

Source: e coli.-derived, PKG

Tag: GST

Domain: 1-221 aa of BC006485

Sequence: MFHGIPATPGIGAPGNKPELYEEVKLYKNAREREKYDNMAELFAVVKTMQALEKAYIKDCVSPSEYTAACSRLLVQYKAAFRQVQGSEISSIDEFCRKFRLDCPLAMERIKEDRPITIKDDKGNLNRCIADVVSLFITVMDKLRLEIRAMDEIQPDLRELMETMHRMSHLPPDFEGRQTVSQWLQTLSGMSASDELDDSQVRQMLFDLESAYNAFNRFLHA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vacuolar protein sorting 28 homolog (S. cerevisiae)
别名 241886D3, ESCRT-I complex subunit VPS28, H-Vps28, Vacuolar protein sorting-associated protein 28 homolog
计算分子量 25 kDa
观测分子量 28 kDa
GenBank蛋白编号BC006485
基因名称 VPS28
Gene ID (NCBI) 51160
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDQ9UK41
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS28 is a subunit of the ESCRT-I complex (endosomal sorting complex I required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. The ESCRT-I complex is composed of VPS23 (also known as TSG101), VPS28, VPS37, and MVB12A or MVB12B. VPS28 interacts with VPS23. Two alternative transcripts encoding different VPS28 isoforms have been described.

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