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验证数据展示

  • WB analysis of mouse brain using 10901-1-AP (same clone as 10901-1-PBS)

    WB analysis of mouse brain using 10901-1-AP (same clone as 10901-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10901-1-AP (VPS18 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 10901-1-AP (same clone as 10901-1-PBS)

    IP experiment of HeLa using 10901-1-AP (same clone as 10901-1-PBS)

    IP result of anti-VPS18 (IP:10901-1-AP, 4ug; Detection:10901-1-AP 1:300) with HeLa cells lysate 920ug. This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 10901-1-AP (same clone as 10901-1-PBS)

    IF Staining of A549 using 10901-1-AP (same clone as 10901-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using VPS18 antibody (10901-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 10901-1-AP (same clone as 10901-1-PBS)

    IHC staining of human lung cancer using 10901-1-AP (same clone as 10901-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung cancer using 10901-1-AP (same clone as 10901-1-PBS)

    IHC staining of human lung cancer using 10901-1-AP (same clone as 10901-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10901-1-AP (VPS18 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10901-1-PBS in a different storage buffer formulation.

VPS18 Polyclonal antibody, PBS Only

VPS18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10901-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

Vacuolar protein sorting-associated protein 18 homolog, KIAA1475, hVPS18

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

10901-1-PBS targets VPS18 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1140

Product name: Recombinant human VPS18 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 226-355 aa of BC001513

Sequence: QRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 18 homolog (S. cerevisiae)
别名 Vacuolar protein sorting-associated protein 18 homolog, KIAA1475, hVPS18
计算分子量 110 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC001513
基因名称 VPS18
Gene ID (NCBI) 57617
RRIDAB_2273089
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P253
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

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