VPS18 Polyclonal antibody

VPS18 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 10901-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, IP, ELISA

Vacuolar protein sorting-associated protein 18 homolog, KIAA1475, hVPS18

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inmouse brain tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue, human testis tissue, mouse brain tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10901-1-AP targets VPS18 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IP
经测试反应性 human, mouse
文献引用反应性human
免疫原 VPS18 fusion protein Ag1140 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 18 homolog (S. cerevisiae)
别名 Vacuolar protein sorting-associated protein 18 homolog, KIAA1475, hVPS18
计算分子量 110 kDa
观测分子量 100-110 kDa
GenBank蛋白编号BC001513
基因名称 VPS18
Gene ID (NCBI) 57617
RRIDAB_2273089
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P253
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Vps18 is a central member of Vps-C complex. Vps18 may play a role in vesicle-mediated protein trafficking to lysosomal compartments and in membrane docking/fusion reactions of late endosomes/lysosomes.

实验方案

Product Specific Protocols
WB protocol for VPS18 antibody 10901-1-APDownload protocol
IHC protocol for VPS18 antibody 10901-1-APDownload protocol
IF protocol for VPS18 antibody 10901-1-APDownload protocol
IP protocol for VPS18 antibody 10901-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
humanWB

Autophagy

Acetylation of STX17 (syntaxin 17) controls autophagosome maturation.

Authors - Qiuhong Shen
humanWB

Curr Biol

Cellular mechanotransduction relies on tension-induced and chaperone-assisted autophagy.

Authors - Anna Ulbricht
humanWB

Int J Mol Sci

Polymeric Polylactic Acid-Glycolic Acid-Based Nanoparticles Deliver Nintedanib Across the Blood-Brain Barrier to Inhibit Glioblastoma Growth

Authors - Ying Dang
humanWB,IP

Nat Commun

Deacetylated SNAP47 recruits HOPS to facilitate autophagosome-lysosome fusion independent of STX17

Authors - Fenglei Jian
humanWB

Autophagy

BAG3 and SYNPO (synaptopodin) facilitate phospho-MAPT/Tau degradation via autophagy in neuronal processes.

Authors - Changyi Ji
Loading...