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验证数据展示

  • WB analysis using 28618-1-AP (same clone as 28618-1-PBS)

    WB analysis using 28618-1-AP (same clone as 28618-1-PBS)

    Various lysates were subjected to Tris-Acetate gel system followed by western blot with 28618-1-AP (VPS13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28618-1-AP (same clone as 28618-1-PBS)

    IHC staining of mouse brain using 28618-1-AP (same clone as 28618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 28618-1-AP (same clone as 28618-1-PBS)

    IHC staining of mouse brain using 28618-1-AP (same clone as 28618-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28618-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

VPS13A Polyclonal antibody, PBS Only

VPS13A Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28618-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

CHAC, Intermembrane lipid transfer protein VPS13A, Vacuolar protein sorting-associated protein 13A

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28618-1-PBS targets VPS13A in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag29532

Product name: Recombinant human VPS13A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 2452-2595 aa of NM_033305

Sequence: PVGSRRLKWRCRKSHGEVTQKDDMMMPIDLGEKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAEQEIAVALQDVGISLVNNYTKQEVAYIGITSSDVVWETKPKKKARWKPMSVKHTEKLEREFKEYTESSPSEDKVI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 13 homolog A (S. cerevisiae)
别名 CHAC, Intermembrane lipid transfer protein VPS13A, Vacuolar protein sorting-associated protein 13A
计算分子量 360 kDa
观测分子量360 kDa
GenBank蛋白编号NM_033305
基因名称 VPS13A
Gene ID (NCBI) 23230
RRIDAB_2918183
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RL7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

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