验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, mouse brain tissue, mouse heart tissue |
| Positive IHC detected in | mouse brain tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
28618-1-AP targets VPS13A in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 |
CatNo: Ag29532 Product name: Recombinant human VPS13A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2452-2595 aa of NM_033305 Sequence: PVGSRRLKWRCRKSHGEVTQKDDMMMPIDLGEKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAEQEIAVALQDVGISLVNNYTKQEVAYIGITSSDVVWETKPKKKARWKPMSVKHTEKLEREFKEYTESSPSEDKVI 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vacuolar protein sorting 13 homolog A (S. cerevisiae) |
| 别名 | CHAC, Chorea acanthocytosis protein, CHOREIN, FLJ42030, KIAA0986, VPS13A |
| 计算分子量 | 360 kDa |
| 观测分子量 | 360 kDa |
| GenBank蛋白编号 | NM_033305 |
| 基因名称 | VPS13A |
| Gene ID (NCBI) | 23230 |
| RRID | AB_2918183 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96RL7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS13A antibody 28618-1-AP | Download protocol |
| IHC protocol for VPS13A antibody 28618-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Analysis of Brain, Blood, and Testis Phenotypes Lacking the Vps13a Gene in C57BL/6N Mice | ||
Cell Death Discov Insights from lipidomics into the terminal maturation of circulating human reticulocytes |
