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验证数据展示

  • WB analysis using 28618-1-AP

    WB analysis using 28618-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28618-1-AP (VPS13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28618-1-AP

    IHC staining of mouse brain using 28618-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28618-1-AP

    IHC staining of mouse brain using 28618-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28618-1-AP (VPS13A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

VPS13A Polyclonal antibody

VPS13A Polyclonal Antibody for WB, IHC, ELISA
Cat No. 28618-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, Mouse and More (1)

应用

WB, IHC, ELISA

别名

CHAC, Chorea acanthocytosis protein, CHOREIN, FLJ42030, KIAA0986, VPS13A

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, mouse heart tissue
Positive IHC detected inmouse brain tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28618-1-AP targets VPS13A in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 Human, Mouse
文献引用反应性mouse
免疫原 VPS13A fusion protein Ag29532 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vacuolar protein sorting 13 homolog A (S. cerevisiae)
别名 CHAC, Chorea acanthocytosis protein, CHOREIN, FLJ42030, KIAA0986, VPS13A
计算分子量 360 kDa
观测分子量360 kDa
GenBank蛋白编号NM_033305
基因名称 VPS13A
Gene ID (NCBI) 23230
RRIDAB_2918183
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96RL7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).

实验方案

Product Specific Protocols
WB protocol for VPS13A antibody 28618-1-APDownload protocol
IHC protocol for VPS13A antibody 28618-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Int J Mol Sci

Analysis of Brain, Blood, and Testis Phenotypes Lacking the Vps13a Gene in C57BL/6N Mice

Authors - Jitrapa Pinyomahakul
View Article