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验证数据展示

  • WB analysis using 84320-2-RR

    WB analysis using 84320-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84320-2-RR (C14orf133 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of NIH/3T3 using 84320-2-RR

    IF Staining of NIH/3T3 using 84320-2-RR

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using VIPAS39 antibody (84320-2-RR, Clone: 241616A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 84320-2-RR

    Affinity and Kinetic Characterization of 84320-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84320-2-RR against Human VIPAS39 were performed. The affinity constant is below 1 pM.

VIPAS39 Recombinant monoclonal antibody

VIPAS39 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 84320-2-RR

产品说明书

CloneNo. 241616A12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

C14orf133, 241616A12, hSPE 39, hSPE-39, SPE39

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, L02 cells, NIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84320-2-RR targets VIPAS39 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14762

Product name: Recombinant human VIPAS39 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 147-493 aa of BC015054

Sequence: EYRDYSNDWSPSDTVRRLRKGKVCSLERFRSLQDKLQLLEEAVSMHDGNVITAVLIFLKRTLSKEILFRELEVRQVALRHLIHFLKEIGDQKLLLDLFRFLDRTEELALSHYREHLNIQDPDKRKEFLKTCVGLPFSAEDSAHIQDHYTLLERQIIIEANDRHLESAGQTEIFRKHPRKASILNMPLVTTLFYSCFYHYTEAEGTFSSPVNLKKTFKIPDKQYVLTALAARAKLRAWNDVDALFTTKNWLGYTKKRAPIGFHRVVEILHKNNAPVQILQEYVNLVEDVDTKLNLATKFKCHDVVIDTYRDLKDRQQLLAYRSKVDKGSAEEEKIDALLSSSQIRWKN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 14 open reading frame 133
别名 C14orf133, 241616A12, hSPE 39, hSPE-39, SPE39
计算分子量 493 aa, 57 kDa
观测分子量50 kDa
GenBank蛋白编号BC015054
基因名称 VIPAS39
Gene ID (NCBI) 63894
RRIDAB_3671861
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9H9C1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).

实验方案

Product Specific Protocols
IF protocol for VIPAS39 antibody 84320-2-RRDownload protocol
WB protocol for VIPAS39 antibody 84320-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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