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Conjugate
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验证数据展示

  • WB analysis using 84320-2-RR (same clone as 84320-2-PBS)

    WB analysis using 84320-2-RR (same clone as 84320-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84320-2-RR (C14orf133 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84320-2-PBS in a different storage buffer formulation.

  • IF Staining of NIH/3T3 using 84320-2-RR (same clone as 84320-2-PBS)

    IF Staining of NIH/3T3 using 84320-2-RR (same clone as 84320-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using VIPAS39 antibody (84320-2-RR, Clone: 241616A12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84320-2-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01194-2

    Cytometric bead array standard curve of MP01194-2

    Cytometric bead array standard curve of MP01194-2, VIPAS39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84320-2-PBS. Detection antibody: 84320-3-PBS. Standard: Ag14762. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP01194-3

    Cytometric bead array standard curve of MP01194-3

    Cytometric bead array standard curve of MP01194-3, VIPAS39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84320-2-PBS. Detection antibody: 84320-1-PBS. Standard: Ag14762. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84320-2-RR

    Affinity and Kinetic Characterization of 84320-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84320-2-RR against Human VIPAS39 were performed. The affinity constant is below 1 pM.

VIPAS39 Recombinant antibody, PBS Only (Capture)

VIPAS39 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 84320-2-PBS

产品说明书

CloneNo. 241616A12

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

C14orf133, 241616A12, hSPE 39, hSPE-39, SPE39

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

84320-2-PBS targets VIPAS39 as part of a matched antibody pair:

MP01194-2: 84320-2-PBS capture and 84320-3-PBS detection (validated in Cytometric bead array)

MP01194-3: 84320-2-PBS capture and 84320-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 VIPAS39 fusion protein Ag14762 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chromosome 14 open reading frame 133
别名 C14orf133, 241616A12, hSPE 39, hSPE-39, SPE39
计算分子量 493 aa, 57 kDa
观测分子量50 kDa
GenBank蛋白编号BC015054
基因名称 VIPAS39
Gene ID (NCBI) 63894
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H9C1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VIPAS39 (also known as C14orf133 or SPE-39) is a binding protein to Vps33B, one of the subunits in the mammalian HOPS complex. VIPAS39 may be involved in endosomal maturation or fusion (PMID: 23918659). Mutations in Vps33B and VIPAS39 cause arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is a rare autosomal recessive multisystem disorder associated with abnormalities in polarized liver and kidney cells (PMID: 20190753).