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验证数据展示

  • WB analysis using 26781-1-AP (same clone as 26781-1-PBS)

    WB analysis using 26781-1-AP (same clone as 26781-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26781-1-AP (VGF antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 26781-1-AP (same clone as 26781-1-PBS)

    IHC staining of human brain using 26781-1-AP (same clone as 26781-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26781-1-AP (VGF Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

  • IP experiment of U2OS using 26781-1-AP (same clone as 26781-1-PBS)

    IP experiment of U2OS using 26781-1-AP (same clone as 26781-1-PBS)

    U2OS lysates prepared and IP of VGF performed using 1.0 μg of 26781-1-AP coupled to protein A- Sepharose beads. The Ponceau stained transfers of each blot are shown. Data provided by YCharOS, an open science company with a mission to validate commercial antibodies to improve scientific reproducibility and transparency. This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

  • IHC staining of human brain using 26781-1-AP (same clone as 26781-1-PBS)

    IHC staining of human brain using 26781-1-AP (same clone as 26781-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 26781-1-AP (VGF Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 26781-1-PBS in a different storage buffer formulation.

VGF Polyclonal antibody, PBS Only

VGF Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 26781-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IP, Indirect ELISA

别名

SCG7, Neurosecretory protein VGF, Neuro-Endocrine Specific Protein VGF, Neuroendocrine regulatory peptide-2, Neuroendocrine regulatory peptide-1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

26781-1-PBS targets VGF in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag24820

Product name: Recombinant human VGF protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 81-280 aa of BC063835

Sequence: ALAAVLLQALDRPASPPAPSGSQQGPEEEAAEALLTETVRSQTHSLPAPESPEPAAPPRPQTPENGPEASDPSEELEALASLLQELRDFSPSSAKRQQETAAAETETRTHTLTRVNLESPGPERVWRASWGEFQARVPERAPLPPPAPSQFQARMPDSGPLPETHKFGEGVSSPKTHLGEALAPLSKAYQGVAAPFPKAR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 VGF nerve growth factor inducible
别名 SCG7, Neurosecretory protein VGF, Neuro-Endocrine Specific Protein VGF, Neuroendocrine regulatory peptide-2, Neuroendocrine regulatory peptide-1
计算分子量 615 aa, 67 kDa
观测分子量 67 kDa, 90-100 kDa
GenBank蛋白编号BC063835
基因名称 VGF
Gene ID (NCBI) 7425
RRIDAB_2880632
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15240
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VGF encodes a neuropeptide precursor with specific expression in a subpopulation of neuroendocrine cells, and it is upregulated by nerve growth factor. The expression of VGF is developmentally regulated in different areas of the brain (PubMed: 18406753). Exercise upregulated expression of VGF in mouse hippocampus, and a synthetic Vgf-derived peptide produced an antidepressant response in mice (PubMed: 18059283). VGF can be detected the precursor form of 90-100 kDa before proteolytic cleavage (PMID:24934694, PMID:10433233).

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