VGF Polyclonal antibody
VGF Polyclonal Antibody for IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IP, WB, ELISA and More (2)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | K-562 cells, mouse brain tissue |
| Positive IP detected in | U2OS cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
26781-1-AP targets VGF in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Immunogen | VGF fusion protein Ag24820 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | VGF nerve growth factor inducible |
| Synonyms | Neurosecretory protein VGF, VGF |
| Calculated Molecular Weight | 615 aa, 67 kDa |
| Observed Molecular Weight | 67 kDa, 90-100 kDa |
| GenBank Accession Number | BC063835 |
| Gene Symbol | VGF |
| Gene ID (NCBI) | 7425 |
| RRID | AB_2880632 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15240 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VGF encodes a neuropeptide precursor with specific expression in a subpopulation of neuroendocrine cells, and it is upregulated by nerve growth factor. The expression of VGF is developmentally regulated in different areas of the brain (PubMed: 18406753). Exercise upregulated expression of VGF in mouse hippocampus, and a synthetic Vgf-derived peptide produced an antidepressant response in mice (PubMed: 18059283). VGF can be detected the precursor form of 90-100 kDa before proteolytic cleavage (PMID:24934694, PMID:10433233).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VGF antibody 26781-1-AP | Download protocol |
| IHC protocol for VGF antibody 26781-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Mol Biol Cell Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites. | ||
Clin Epigenetics METTL3 exerts synergistic effects on m6A methylation and histone modification to regulate the function of VGF in lung adenocarcinoma | ||
J Exp Clin Cancer Res Hedgehog-Gli1-derived exosomal circ-0011536 mediates peripheral neural remodeling in pancreatic cancer by modulating the miR-451a/VGF axis |