验证数据展示
经过测试的应用
Positive WB detected in | K-562 cells, mouse brain tissue |
Positive IP detected in | U2OS cells |
Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
26781-1-AP targets VGF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IP, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse |
文献引用反应性 | human |
免疫原 | VGF fusion protein Ag24820 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | VGF nerve growth factor inducible |
别名 | SCG7, Neurosecretory protein VGF, Neuro-Endocrine Specific Protein VGF, Neuroendocrine regulatory peptide-2, Neuroendocrine regulatory peptide-1 |
计算分子量 | 615 aa, 67 kDa |
观测分子量 | 67 kDa, 90-100 kDa |
GenBank蛋白编号 | BC063835 |
基因名称 | VGF |
Gene ID (NCBI) | 7425 |
RRID | AB_2880632 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O15240 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VGF encodes a neuropeptide precursor with specific expression in a subpopulation of neuroendocrine cells, and it is upregulated by nerve growth factor. The expression of VGF is developmentally regulated in different areas of the brain (PubMed: 18406753). Exercise upregulated expression of VGF in mouse hippocampus, and a synthetic Vgf-derived peptide produced an antidepressant response in mice (PubMed: 18059283). VGF can be detected the precursor form of 90-100 kDa before proteolytic cleavage (PMID:24934694, PMID:10433233).
实验方案
Product Specific Protocols | |
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WB protocol for VGF antibody 26781-1-AP | Download protocol |
IHC protocol for VGF antibody 26781-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Mol Biol Cell Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites. | ||
Clin Epigenetics METTL3 exerts synergistic effects on m6A methylation and histone modification to regulate the function of VGF in lung adenocarcinoma | ||
J Exp Clin Cancer Res Hedgehog-Gli1-derived exosomal circ-0011536 mediates peripheral neural remodeling in pancreatic cancer by modulating the miR-451a/VGF axis |