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验证数据展示

  • WB analysis using 17684-1-AP (same clone as 17684-1-PBS)

    WB analysis using 17684-1-AP (same clone as 17684-1-PBS)

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

    IHC staining of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

    IHC staining of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

    IP experiment of mouse brain using 17684-1-AP (same clone as 17684-1-PBS)

    IP result of anti-VEZT (IP:17684-1-AP, 4ug; Detection:17684-1-AP 1:500) with mouse brain tissue lysate 4400ug. This data was developed using the same antibody clone with 17684-1-PBS in a different storage buffer formulation.

VEZT Polyclonal antibody, PBS Only

VEZT Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 17684-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17684-1-PBS targets VEZT in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11786

Product name: Recombinant human VEZT protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 382-731 aa of BC064939

Sequence: VRSLQLHLKALLNEVIILEDELEKLVCTKETQELVSEAYPILEQKLKLIQPHVQASNNCWEEAISQVDKLLRRNTDKKGKPEIACENPHCTVVPLKQPTLHIADKDPIPEEQELEAYVDDIDIDSDFRKDDFYYLSQEDKERQKREHEESKRVLQELKSVLGFKASEAERQKWKQLLFSDHAVLKSLSPVDPVEPISNSEPSMNSDMGKVSKNDTEEESNKSATTDNEISRTEYLCENALEGKNKDNSSNEVFPQGAEERMCYQCESEDEPQADGSGLTTAPPTPRDSLQPSIKQRLARLQLSPDFTFTAGLAAEVAARSLSFTTMQEQTFGDEEEEQIIEENKNEIEEK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vezatin, adherens junctions transmembrane protein
别名
计算分子量 731 aa, 83 kDa
观测分子量 89 kDa, 125 kDa
GenBank蛋白编号BC064939
基因名称 VEZT
Gene ID (NCBI) 55591
RRIDAB_2213139
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBM0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Vezatin (VEZT) is an adherens junctions transmembrane protein which identified as a putative tumor suppressor. In case of Listeria infection, it promotes bacterial internalization by participating in myosin VIIa recruitment to the entry site. There're two isoforms with calculated MW 89 kDa and 65 kDa. With modification or glycosylation, the MW in Western blot detection will be presented as ~125 kDa and ~89 kDa (PMID: 11080149) or 65-71 kDa and 88-100 kDa (PMID: 20049712). There're some isoforms with MW less than 70 kDa.

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