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验证数据展示

  • WB analysis using 17684-1-AP

    WB analysis using 17684-1-AP

    mouse eye tissue were subjected to SDS PAGE followed by western blot with 17684-1-AP (VEZT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 17684-1-AP

    IHC staining of mouse brain using 17684-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 17684-1-AP

    IHC staining of mouse brain using 17684-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17684-1-AP (VEZT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of mouse brain using 17684-1-AP

    IP experiment of mouse brain using 17684-1-AP

    IP result of anti-VEZT (IP:17684-1-AP, 4ug; Detection:17684-1-AP 1:500) with mouse brain tissue lysate 4400ug.

VEZT Polyclonal antibody

VEZT Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 17684-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA

别名

VEZATIN, VEZT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse eye tissue, human brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17684-1-AP targets VEZT in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 VEZT fusion protein Ag11786 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vezatin, adherens junctions transmembrane protein
别名 VEZATIN, VEZT
计算分子量 731 aa, 83 kDa
观测分子量 89 kDa, 125 kDa
GenBank蛋白编号BC064939
基因名称 VEZT
Gene ID (NCBI) 55591
RRIDAB_2213139
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HBM0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Vezatin (VEZT) is an adherens junctions transmembrane protein which identified as a putative tumor suppressor. In case of Listeria infection, it promotes bacterial internalization by participating in myosin VIIa recruitment to the entry site. There're two isoforms with calculated MW 89 kDa and 65 kDa. With modification or glycosylation, the MW in Western blot detection will be presented as ~125 kDa and ~89 kDa (PMID: 11080149) or 65-71 kDa and 88-100 kDa (PMID: 20049712). There're some isoforms with MW less than 70 kDa.

实验方案

Product Specific Protocols
WB protocol for VEZT antibody 17684-1-APDownload protocol
IHC protocol for VEZT antibody 17684-1-APDownload protocol
IP protocol for VEZT antibody 17684-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols