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验证数据展示

  • IF Staining of MCF-7 using CL488-83727-7

    IF Staining of MCF-7 using CL488-83727-7

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 VEGF-C antibody (CL488-83727-7, Clone: 251992H1 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated VEGF-C Recombinant monoclonal antibody

VEGF-C Uni-rAb® Recombinant Antibody for IF/ICC
Cat No. CL488-83727-7

产品说明书

CloneNo. 251992H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

VEGFC, vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, VEGF C

缓冲液配方:  PBS, Proclin300, BSA, Glycerol
PBS, Proclin300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy VEGFC Ready-To-Use IHC Kit. VEGFC primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 83727-7-PBS

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83727-7 targets VEGF-C in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Eg1482

Product name: Recombinant human VEGFC protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 112-227 aa of BC035212

Sequence: AHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGVATNTFFKPPCVSVYRCGGCCNSEGLQCMNTSTSYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIRR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vascular endothelial growth factor C
别名 VEGFC, vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, VEGF C
计算分子量 419 aa, 47 kDa
观测分子量43 kDa
GenBank蛋白编号BC035212
基因名称 VEGFC
Gene ID (NCBI) 7424
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
激发激光Blue laser (488 nm)
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49767
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFC is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. The main function of VEGFC is to promote the growth of lymphatic vessels (lymphangiogenesis). It acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth, and migration.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 VEGF-C antibody CL488-83727-7Download protocol
Standard Protocols
Click here to view our Standard Protocols
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