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验证数据展示

  • WB analysis using 83727-7-RR

    WB analysis using 83727-7-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83727-7-RR (VEGF-C antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of MCF-7 using 83727-7-RR

    IF Staining of MCF-7 using 83727-7-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using VEGF-C antibody (83727-7-RR, Clone: 251992H1 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Affinity and Kinetic Characterization of 83727-7-RR

    Affinity and Kinetic Characterization of 83727-7-RR

    Biolayer interferometry (BLl) kinetic assays of 83727-7-RR against Human VEGF-C were performed. The affinity constant is below 1 pM.

VEGF-C Recombinant monoclonal antibody

VEGF-C Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83727-7-RR

产品说明书

CloneNo. 251992H1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

VEGFC, vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, VEGF C

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells, SW480 cells
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy VEGFC Ready-To-Use IHC Kit. VEGFC primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83727-7-RR targets VEGF-C in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Eg1482

Product name: Recombinant human VEGFC protein

Source: mammalian cells-derived, pHZ-KIsec-C-rFc

Tag: C-rFc

Domain: 112-227 aa of BC035212

Sequence: AHYNTEILKSIDNEWRKTQCMPREVCIDVGKEFGVATNTFFKPPCVSVYRCGGCCNSEGLQCMNTSTSYLSKTLFEITVPLSQGPKPVTISFANHTSCRCMSKLDVYRQVHSIIRR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vascular endothelial growth factor C
别名 VEGFC, vascular endothelial growth factor C, Vascular endothelial growth factor-related protein, VEGF C
计算分子量 419 aa, 47 kDa
观测分子量43 kDa
GenBank蛋白编号BC035212
基因名称 VEGFC
Gene ID (NCBI) 7424
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49767
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VEGFC is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. The main function of VEGFC is to promote the growth of lymphatic vessels (lymphangiogenesis). It acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth, and migration.

实验方案

Product Specific Protocols
IF protocol for VEGF-C antibody 83727-7-RRDownload protocol
WB protocol for VEGF-C antibody 83727-7-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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