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验证数据展示

  • WB analysis using 27956-1-AP (same clone as 27956-1-PBS)

    WB analysis using 27956-1-AP (same clone as 27956-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27956-1-AP (VE-cadherin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27956-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 27956-1-AP (same clone as 27956-1-PBS)

    IHC staining of human placenta using 27956-1-AP (same clone as 27956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27956-1-AP (VE-cadherin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27956-1-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 27956-1-AP (same clone as 27956-1-PBS)

    IHC staining of human placenta using 27956-1-AP (same clone as 27956-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27956-1-AP (VE-cadherin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27956-1-PBS in a different storage buffer formulation.

  • FC experiment of HUVEC using 27956-1-AP (same clone as 27956-1-PBS)

    FC experiment of HUVEC using 27956-1-AP (same clone as 27956-1-PBS)

    1X10^6 HUVEC cells were surface stained with 0.4 ug Anti-Human VE-cadherin (27956-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000, or 0.4 ug Control Antibody. Cells were not fixed. This data was developed using the same antibody clone with 27956-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 27956-1-AP (same clone as 27956-1-PBS)

    IF Staining of HUVEC using 27956-1-AP (same clone as 27956-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using VE-cadherin/CD144 antibody (27956-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 27956-1-PBS in a different storage buffer formulation.

  • IF Staining of HUVEC using 27956-1-AP (same clone as 27956-1-PBS)

    IF Staining of HUVEC using 27956-1-AP (same clone as 27956-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using VE-cadherin/CD144 antibody (27956-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 27956-1-PBS in a different storage buffer formulation.

VE-cadherin/CD144 Polyclonal antibody, PBS Only

VE-cadherin/CD144 Polyclonal Antibody for WB, IHC, IF/ICC, FC, Indirect ELISA
Cat No. 27956-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC, Indirect ELISA

别名

VE-cadherin, 7B4, 7B4 antigen, Cadherin 5, Cadherin-5

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

27956-1-PBS targets VE-cadherin/CD144 in WB, IHC, IF/ICC, FC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag27487

Product name: Recombinant human CDH5 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 54-241 aa of NM_001795

Sequence: MHIDEEKNTSLPHHVGKIKSSVSRKNAKYLLKGEYVGKVFRVDAETGDVFAIERLDRENISEYHLTAVIVDKDTGENLETPSSFTIKVHDVNDNWPVFTHRLFNASVPESSAVGTSVISVTAVDADDPTVGDHASVMYQILKGKEYFAIDNSGRIITITKSLDREKQARYEIVVEARDAQGLRGDSGT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cadherin 5, type 2 (vascular endothelium)
别名 VE-cadherin, 7B4, 7B4 antigen, Cadherin 5, Cadherin-5
计算分子量 88 kDa
观测分子量120-140 kDa
GenBank蛋白编号NM_001795
基因名称 VE-cadherin
Gene ID (NCBI) 1003
RRIDAB_2918136
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP33151
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609). The calculated molecular weight of VE-cadherin is 88 kDa and the apparent molecular weight of 120-140 kDa is higher due to post-translational glycosylation and phosphorylation (PMID: 10460833; 29894844). Full-length VE-cadherin can be proteolytically cleaved to generate a fragment of 90-100 kDa (PMID: 9786462; 22064597).

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