VE-cadherin Polyclonal antibody
VE-cadherin Polyclonal Antibody for FC, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human and More (1)
Applications
FC, IHC, WB, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | HUVEC cells, human placenta tissue |
Positive IHC detected in | human placenta tissue, human breast cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HUVEC cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:4000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 6 publications below |
IF | See 7 publications below |
产品信息
27956-1-AP targets VE-cadherin in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human samples.
Tested Applications | FC, IHC, WB, ELISA |
Cited Applications | WB, IF |
Tested Reactivity | Human |
Cited Reactivity | human |
Immunogen | VE-cadherin fusion protein Ag27487 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | cadherin 5, type 2 (vascular endothelium) |
Synonyms | 7B4, 7B4 antigen, Cadherin 5, CD144, CDH5, FLJ17376, Vascular endothelial cadherin, VE cadherin |
Calculated Molecular Weight | 88 kDa |
Observed Molecular Weight | 120-140 kDa |
GenBank Accession Number | NM_001795 |
Gene Symbol | CDH5 |
Gene ID (NCBI) | 1003 |
RRID | AB_2918136 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P33151 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for VE-cadherin antibody 27956-1-AP | Download protocol |
IHC protocol for VE-cadherin antibody 27956-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nat Commun FNIP1 abrogation promotes functional revascularization of ischemic skeletal muscle by driving macrophage recruitment | ||
Bioengineering (Basel) Organotypic 3D Co-Culture of Human Pleura as a Novel In Vitro Model of Staphylococcus aureus Infection and Biofilm Development | ||
Front Cell Infect Microbiol Analysis of miRNAs Involved in Mouse Heart Injury Upon Coxsackievirus A2 Infection. | ||
Bioengineering (Basel) A Transwell-Based Vascularized Model to Investigate the Effect of Interstitial Flow on Vasculogenesis | ||
J Hypertens Short-chain acyl-CoA dehydrogenase is a potential target for the treatment of vascular remodelling | ||
Oral Dis Artemisinin suppressed tumour growth and induced vascular normalisation in oral squamous cell carcinoma via inhibition of MIF |