CoraLite®594-conjugated VE-cadherin Monoclonal antibody
VE-cadherin Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human and More (1)
Applications
IF-P and More (1)
Conjugate
CoraLite®594 Fluorescent Dye
CloneNo.
4F9B10
验证数据展示
经过测试的应用
| Positive IF detected in | human breast cancer tissue, human tonsillitis tissue |
For other applications, we recommend the unconjugated version of this antibody, 66804-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 2 publications below |
产品信息
CL594-66804 targets VE-cadherin in WB, IF applications and shows reactivity with Human samples.
| Tested Applications | IF-P |
| Cited Applications | WB, IF |
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Immunogen | VE-cadherin fusion protein Ag27501 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cadherin 5, type 2 (vascular endothelium) |
| Synonyms | 7B4, 7B4 antigen, Cadherin 5, CD144, CDH5, FLJ17376, Vascular endothelial cadherin, VE cadherin, VE-cadherin |
| Calculated Molecular Weight | 88 kDa |
| GenBank Accession Number | NM_001795 |
| Gene Symbol | CDH5 |
| Gene ID (NCBI) | 1003 |
| RRID | AB_2920043 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P33151 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). E-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 VE-cadherin antibody CL594-66804 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Biochem Biophys Res Commun Irisin links Claudin-5 preservation and Mfn2-mediated mitochondrial dynamics to resist doxorubicin-induced cardiac endothelial damage | ||
Biomacromolecules Interpenetrating Polymer Network HA/Alg-RGD Hydrogel: An Equilibrium of Macroscopic Stability and Microscopic Adaptability for 3D Cell Growth and Vascularization |
