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CoraLite® Plus 488-conjugated VDAC2 Monoclonal antibody

VDAC2 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1D8C11

Cat No : CL488-66388

Print datasheet

Synonyms

FLJ23841, hVDAC2, VDAC 2, VDAC2

验证数据展示

  • FC experiment of HEK-293 using CL488-66388

    FC experiment of HEK-293 using CL488-66388

    1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human VDAC2 (CL488-66388, Clone:1D8C11) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL488-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 66388-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66388 targets VDAC2 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name voltage-dependent anion channel 2
Synonyms FLJ23841, hVDAC2, VDAC 2, VDAC2
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 30-33 kDa
GenBank Accession NumberNM_003375
Gene Symbol VDAC2
Gene ID (NCBI) 7417
RRIDAB_2919319
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP45880
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

VDAC2 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 VDAC2 antibody CL488-66388Download protocol
Standard Protocols
Click here to view our Standard Protocols