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VCP Recombinant antibody

VCP Recombinant Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

3G23

Cat No : 82463-1-RR

Print datasheet

Synonyms

15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP

验证数据展示

  • WB analysis using 82463-1-RR

    WB analysis using 82463-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82463-1-RR (VCP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of rat small intestine using 82463-1-RR

    IHC staining of rat small intestine using 82463-1-RR

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 82463-1-RR (VCP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with VCP antibody included

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, MCF-7 cells, RAW 264.7 cells, human brain tissue, mouse brain tissue, rat brain tissue
Positive IHC detected inrat small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy VCP Ready-To-Use IHC Kit. VCP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82463-1-RR targets VCP in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Tested Reactivity Human, mouse, rat
Immunogen VCP fusion protein Ag1002 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name valosin-containing protein
Synonyms 15S Mg(2+) ATPase p97 subunit, IBMPFD, p97, TER ATPase, TERA, valosin containing protein, VCP
Calculated Molecular Weight 89 kDa
Observed Molecular Weight89 kDa
GenBank Accession NumberBC007562
Gene Symbol VCP
Gene ID (NCBI) 7415
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP55072
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

实验方案

Product Specific Protocols
WB protocol for VCP antibody 82463-1-RRDownload protocol
IHC protocol for VCP antibody 82463-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols