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验证数据展示

  • WB analysis using 82463-1-RR (same clone as 82463-1-PBS)

    WB analysis using 82463-1-RR (same clone as 82463-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82463-1-RR (VCP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 82463-1-RR (same clone as 82463-1-PBS)

    IP experiment of HeLa using 82463-1-RR (same clone as 82463-1-PBS)

    IP result of anti-VCP (IP:82463-1-RR, 4ug; Detection:82463-1-RR 1:2000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

  • IHC staining of rat small intestine using 82463-1-RR (same clone as 82463-1-PBS)

    IHC staining of rat small intestine using 82463-1-RR (same clone as 82463-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 82463-1-RR (VCP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VCP antibody included
  • IF Staining of rat brain using 82463-1-RR (same clone as 82463-1-PBS)

    IF Staining of rat brain using 82463-1-RR (same clone as 82463-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:500 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 82463-1-RR (same clone as 82463-1-PBS)

    IF Staining of HeLa using 82463-1-RR (same clone as 82463-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using VCP antibody (82463-1-RR, Clone: 3G23 ) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 82463-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82463-1-RR

    Affinity and Kinetic Characterization of 82463-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82463-1-RR against Human VCP were performed. The affinity constant is 0.997 nM.

VCP Recombinant monoclonal antibody, PBS Only

VCP Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA
Cat No. 82463-1-PBS

产品说明书

CloneNo. 3G23

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, Indirect ELISA

别名

HEL-S-70, HEL-220, EC:3.6.4.6, 3G23, 15S Mg(2+)-ATPase p97 subunit

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy VCP Ready-To-Use IHC Kit. VCP primary antibody included.

产品信息

82463-1-PBS targets VCP in WB, IHC, IF/ICC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1002

Product name: Recombinant human VCP protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 345-644 aa of BC007562

Sequence: GMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 valosin-containing protein
别名 HEL-S-70, HEL-220, EC:3.6.4.6, 3G23, 15S Mg(2+)-ATPase p97 subunit
计算分子量 89 kDa
观测分子量89 kDa
GenBank蛋白编号BC007562
基因名称 VCP
Gene ID (NCBI) 7415
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP55072
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VCP(Valosin-containing protein), also known as TER ATPase and 15S Mg2+-ATPase p97 subunit, belongs to the AAA ATPase family. VCP was first identified as a result of attempts to clone a putative peptide hormone called valosin. It was found that the cloned cDNA encoded a ubiquitously expressed 90 kDa cytosolic protein, termed VCP, which showed none of the characteristics of a peptide hormone precursor(PMID:1382975). Defects in VCP are the cause of inclusion body myopathy with early-onset Paget disease and frontotemporal dementia (IBMPFD) and amyotrophic lateral sclerosis type 14 with or without frontotemporal dementia (ALS14). VCP has a calculated molecular weight of 89 kDa and an apparent molecular weight of 90-100 kDa (PMID: 15732117, 1382975).

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