验证数据展示
经过测试的应用
| Positive IF/ICC detected in | SKOV-3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-67753 targets VASH2 in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | VASH2 fusion protein Ag29985 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | vasohibin 2 |
| 别名 | FLJ12505, RP11 275G3.1, VASH2, VASHL, vasohibin 2, Vasohibin like protein |
| 计算分子量 | 355 aa, 40 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC028194 |
| 基因名称 | VASH2 |
| Gene ID (NCBI) | 79805 |
| RRID | AB_2919537 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q86V25 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VASH2, also named as vasohibin 2, is an important pro-angiogenesis factor in solid tumor. It has been reported that VASH2 is expressed in mononuclear cells mobilized from bone marrow to promote angiogenesis. VASH2 also plays a key role in axon formation. VASH2 is localized as a nuclear type(with 311 amino acid residues) and cytoplasmic type (with 355 amino acid residues and low abundance). Cytoplasmic VASH2 is associated with carcinoma angiogenesis, while nuclear VASH2 may be associated with cell proliferation. 67753-1-Ig antibody recognizes both the nuclear and cytoplasmic isoforms. (PMID:23615928; 19204325; 31235911; 26177649)
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 VASH2 antibody CL488-67753 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |