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验证数据展示

  • WB analysis using 85400-4-RR (same clone as 85400-4-PBS)

    WB analysis using 85400-4-RR (same clone as 85400-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85400-4-RR (VAMP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85400-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 85400-4-RR (same clone as 85400-4-PBS)

    IHC staining of human placenta using 85400-4-RR (same clone as 85400-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85400-4-RR (VAMP8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85400-4-PBS in a different storage buffer formulation.

  • IHC staining of human placenta using 85400-4-RR (same clone as 85400-4-PBS)

    IHC staining of human placenta using 85400-4-RR (same clone as 85400-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85400-4-RR (VAMP8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85400-4-PBS in a different storage buffer formulation.

  • IF Staining of THP-1 using 85400-4-RR (same clone as 85400-4-PBS)

    IF Staining of THP-1 using 85400-4-RR (same clone as 85400-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using VAMP8 antibody (85400-4-RR, Clone: 242830B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85400-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85400-4-RR

    Affinity and Kinetic Characterization of 85400-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85400-4-RR against Human VAMP8 were performed. The affinity constant is 0.631 nM.

VAMP8 Recombinant monoclonal antibody, PBS Only

VAMP8 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85400-4-PBS

产品说明书

CloneNo. 242830B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

VAMP-8, 242830B11, EDB, Endobrevin, VAMP 8

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

85400-4-PBS targets VAMP8 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7903

Product name: Recombinant human VAMP8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-81 aa of BC001634

Sequence: MEEASEGGGNDRVRNLQSEVEGVKNIMTQNVERILARGENLEHLRNKTEDLEATSEHFKTTSQKVARKFWWKNVKMIVLIC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vesicle-associated membrane protein 8 (endobrevin)
别名 VAMP-8, 242830B11, EDB, Endobrevin, VAMP 8
计算分子量 11 kDa
观测分子量 15 kDa
GenBank蛋白编号BC001634
基因名称 VAMP8
Gene ID (NCBI) 8673
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BV40
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.

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