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验证数据展示

  • WB analysis using 85400-4-RR

    WB analysis using 85400-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85400-4-RR (VAMP8 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 85400-4-RR

    IHC staining of human placenta using 85400-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85400-4-RR (VAMP8 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human placenta using 85400-4-RR

    IHC staining of human placenta using 85400-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85400-4-RR (VAMP8 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of THP-1 using 85400-4-RR

    IF Staining of THP-1 using 85400-4-RR

    Immunofluorescent analysis of (4% PFA) fixed THP-1 cells using VAMP8 antibody (85400-4-RR, Clone: 242830B11 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85400-4-RR

    Affinity and Kinetic Characterization of 85400-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85400-4-RR against Human VAMP8 were performed. The affinity constant is 0.631 nM.

VAMP8 Recombinant antibody

VAMP8 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85400-4-RR

产品说明书

CloneNo. 242830B11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

VAMP-8, 242830B11, EDB, Endobrevin, VAMP 8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inTHP-1 cells, HL-60 cells, A431 cells, HeLa cells, rat kidney tissue, mouse kidney tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inTHP-1 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85400-4-RR targets VAMP8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 VAMP8 fusion protein Ag7903 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 vesicle-associated membrane protein 8 (endobrevin)
别名 VAMP-8, 242830B11, EDB, Endobrevin, VAMP 8
计算分子量 11 kDa
观测分子量 15 kDa
GenBank蛋白编号BC001634
基因名称 VAMP8
Gene ID (NCBI) 8673
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BV40
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

VAMP8, also named as endobrevin, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family and the SNARE (soluble NSF-attachment protein receptor) superfamily. Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP8 is involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. It is required for dense-granule secretion in platelets and plays a role in regulated enzyme secretion in pancreatic acinar cells. VAMP8 is also involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells.

实验方案

Product Specific Protocols
WB protocol for VAMP8 antibody 85400-4-RRDownload protocol
IHC protocol for VAMP8 antibody 85400-4-RRDownload protocol
IF protocol for VAMP8 antibody 85400-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols