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验证数据展示

  • WB analysis of pig brain using 66488-1-Ig (same clone as 66488-1-PBS)

    WB analysis of pig brain using 66488-1-Ig (same clone as 66488-1-PBS)

    Pig brain, rat brain and mouse brain tissues were subjected to SDS PAGE followed by western blot with 66488-1-Ig (VAMP3/Cellubrevin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 66488-1-Ig (same clone as 66488-1-PBS)

    IF Staining of Neuro-2a using 66488-1-Ig (same clone as 66488-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed Neuro-2a cells using VAMP3/Cellubrevin antibody (66488-1-Ig, Clone: 1D10D10 ) at dilution of 1:1800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66488-1-PBS in a different storage buffer formulation.

VAMP3/Cellubrevin Monoclonal antibody, PBS Only

VAMP3/Cellubrevin Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 66488-1-PBS

产品说明书

CloneNo. 1D10D10

宿主/亚型

Mouse / IgG2b

种属反应性

human, mouse, rat, pig

应用

WB, IF/ICC, Indirect ELISA

别名

VAMP3, Synaptobrevin-3, SYB3, Cellubrevin, CEB

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy VAMP3/Cellubrevin Ready-To-Use IHC Kit. VAMP3/Cellubrevin primary antibody included.

产品信息

66488-1-PBS targets VAMP3/Cellubrevin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原

CatNo: Ag1156

Product name: Recombinant human VAMP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-99 aa of BC005941

Sequence: MSTGPTAATGSNRRLQQTQNQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNCEMWAIGITVLVIFIIIIIVWVVS

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 vesicle-associated membrane protein 3 (cellubrevin)
别名 VAMP3, Synaptobrevin-3, SYB3, Cellubrevin, CEB
计算分子量 11 kDa
观测分子量 17 kDa
GenBank蛋白编号BC005941
基因名称 VAMP3
Gene ID (NCBI) 9341
RRIDAB_2881853
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ15836
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VAMP3, also named cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.