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验证数据展示

  • WB analysis of HEK-293 using 10702-1-AP (same clone as 10702-1-PBS)

    WB analysis of HEK-293 using 10702-1-AP (same clone as 10702-1-PBS)

    WB result of VAMP3/Cellubrevin antibody (10702-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-VAMP3/Cellubrevin transfected HEK-293 cells. This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 10702-1-AP (same clone as 10702-1-PBS)

    WB analysis of HEK-293 using 10702-1-AP (same clone as 10702-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

  • IP experiment of HEK-293 using 10702-1-AP (same clone as 10702-1-PBS)

    IP experiment of HEK-293 using 10702-1-AP (same clone as 10702-1-PBS)

    IP result of anti-VAMP3/Cellubrevin (IP:10702-1-AP, 4ug; Detection:10702-1-AP 1:1000) with HEK-293 cells lysate 3000ug. This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 10702-1-AP (same clone as 10702-1-PBS)

    IHC staining of mouse testis using 10702-1-AP (same clone as 10702-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 10702-1-AP (VAMP3/Cellubrevin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10702-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with VAMP3/Cellubrevin antibody included
  • Featured Product
  • KD/KO Validated

VAMP3/Cellubrevin Polyclonal antibody, PBS Only

VAMP3/Cellubrevin Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 10702-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

VAMP3, CEB, Cellubrevin, SYB3, Synaptobrevin-3

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy VAMP3/Cellubrevin Ready-To-Use IHC Kit. VAMP3/Cellubrevin primary antibody included.

产品信息

10702-1-PBS targets VAMP3/Cellubrevin in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1156

Product name: Recombinant human VAMP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-99 aa of BC005941

Sequence: MSTGPTAATGSNRRLQQTQNQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNCEMWAIGITVLVIFIIIIIVWVVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 vesicle-associated membrane protein 3 (cellubrevin)
别名 VAMP3, CEB, Cellubrevin, SYB3, Synaptobrevin-3
计算分子量 11 kDa
观测分子量 11-17 kDa
GenBank蛋白编号BC005941
基因名称 VAMP3
Gene ID (NCBI) 9341
RRIDAB_2212628
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ15836
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.

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