验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, MCF-7 cells, mouse brain tissue |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human heart tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10702-1-AP targets VAMP3/Cellubrevin in WB, IHC, IP, ELISA, Blocking, IF applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, Blocking, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | VAMP3/Cellubrevin fusion protein Ag1156 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | vesicle-associated membrane protein 3 (cellubrevin) |
| 别名 | CEB, Cellubrevin, SYB3, Synaptobrevin 3, VAMP 3, VAMP3 |
| 计算分子量 | 11 kDa |
| 观测分子量 | 11-17 kDa |
| GenBank蛋白编号 | BC005941 |
| 基因名称 | VAMP3 |
| Gene ID (NCBI) | 9341 |
| RRID | AB_2212628 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15836 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
VAMP3, also named as cellubrevin or synaptobrevin 3, is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. VAMP3 is a v-SNARE (soluble NSF-attachment protein receptor). Characterized by a common sequence called the SNARE motif, SNARE proteins are involved in membrane fusion and vesicular transport (PMID: 11252968). VAMP3 resides in recycling endosomes and endosome-derived transport vesicles, involved in regulating membrane traffic. It is implicated in recycling of transferrin receptors, secretion of alpha-granules in platelets, and membrane trafficking during cell migration.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for VAMP3/Cellubrevin antibody 10702-1-AP | Download protocol |
| IHC protocol for VAMP3/Cellubrevin antibody 10702-1-AP | Download protocol |
| IP protocol for VAMP3/Cellubrevin antibody 10702-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) Engineered Cell-Derived Microparticles Bi2Se3/DOX@MPs for Imaging Guided Synergistic Photothermal/Low-Dose Chemotherapy of Cancer. | ||
Adv Sci (Weinh) Early Endosomes Act as Local Exocytosis Hubs to Repair Endothelial Membrane Damage | ||
Aging Cell Knockdown of astrocytic Grin2a aggravates β-amyloid-induced memory and cognitive deficits through regulating nerve growth factor. | ||
Oncotarget A novel liver metastasis-correlated protein of pancreatic neuroendocrine neoplasm (PanNEN) discovered by proteomic analysis. | ||
Front Cell Dev Biol VAMP3 and SNAP23 as Potential Targets for Preventing the Disturbed Flow-Accelerated Thrombus Formation. |
