V5-Trap™ Agarose

V5-Trap™ Agarose is an affinity reagent for IP and purification of V5-tagged proteins. It consists of an anti-V5 Nanobody/ VHH coupled to agarose beads.

Catalog No:
v5ta

Applications:
IP, CoIP, ChIP, RIP

Clonality:
Monoclonal

Host:
Alpaca

Affinity:
Dissociation constant KD of 40 nM

Specificity/Target:
V5-tag sequence GKPIPNPLLGLDST at the N-terminus, C-terminus, or internal site of the fusion protein.

Coupled VHH:
Recombinant, monoclonal anti-V5-tag single domain antibody (sdAb) fragment

Conjugate:
Agarose

Beads:
~ 90 µm (cross-linked 4 % agarose beads)

Binding capacity:
10 µL slurry bind about 6 µg of recombinant V5-tagged protein (~30 kDa)

Elution buffer:
V5-peptide
SDS sample buffer
0.1 M glycine pH 2.0

Storage Buffer:
20% EtOH

Storage Condition:
Shipped at ambient temperature. Upon receipt store at 4°C. Stable for one year.

Synonyms:
V5 tag, V5, GKPIPNPLLGLDST, V5-Trap

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