验证数据展示
经过测试的应用
| Positive WB detected in | mouse kidney tissue |
| Positive IHC detected in | mouse kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
85597-4-RR targets Uromodulin in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse samples.
| 经测试应用 | WB, IHC, IF-P, ELISA Application Description |
| 经测试反应性 | mouse |
| 免疫原 | Uromodulin fusion protein Eg2748 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | Uromodulin |
| 别名 | Tamm-Horsfall urinary glycoprotein, THP, Umod, Uromodulin, secreted form |
| 计算分子量 | 71 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | NM_009470.5 |
| 基因名称 | Umod |
| Gene ID (NCBI) | 22242 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q91X17 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Uromodulin, also known as THP (Tamm-Horsfall glycoprotein), is the most abundant protein excreted in the urine under physiological conditions. Uromodulin is synthesized by the kidney and localizes in cells lining the ascending limb of Henle and distal convoluted tubule. Uromodulin is linked to water/electrolyte balance and kidney innate immunity. Also, studies in knockout mice demonstrated that it has a protective role against urinary tract infections and renal stone formation. Mutations in the gene encoding uromodulin lead to rare autosomal dominant diseases, collectively referred to as uromodulin-associated kidney diseases. (PMID: 21654721)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Uromodulin antibody 85597-4-RR | Download protocol |
| IHC protocol for Uromodulin antibody 85597-4-RR | Download protocol |
| IF protocol for Uromodulin antibody 85597-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |