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验证数据展示

  • WB analysis using 26707-1-AP (same clone as 26707-1-PBS)

    WB analysis using 26707-1-AP (same clone as 26707-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 26707-1-AP (USP54 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26707-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 26707-1-AP (same clone as 26707-1-PBS)

    IHC staining of mouse liver using 26707-1-AP (same clone as 26707-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26707-1-AP (USP54 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26707-1-PBS in a different storage buffer formulation.

USP54 Polyclonal antibody, PBS Only

USP54 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 26707-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

C10orf29, Inactive ubiquitin carboxyl-terminal hydrolase 54, Inactive ubiquitin-specific peptidase 54

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

26707-1-PBS targets USP54 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25080

Product name: Recombinant human USP54 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 377-725 aa of BC130633

Sequence: DSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQHSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 54
别名 C10orf29, Inactive ubiquitin carboxyl-terminal hydrolase 54, Inactive ubiquitin-specific peptidase 54
观测分子量180 kDa
GenBank蛋白编号BC130633
基因名称 USP54
Gene ID (NCBI) 159195
RRIDAB_3669564
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ70EL1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP54 is a ubiquitin-specific protease in the deubiquitinating enzyme (DUB) family that promotes the malignant progression of many cancers. As a deubiquitinating enzyme, USP54 maintains the stability of PLK4 protein through deubiquitination, which promotes centrosome amplification and GC progression. USP54 is also highly expressed in colorectal cancers, and is associated with proliferation, invasion and migration of colorectal cancer. In addition, USP54 plays a key role in the transformation of hormone-sensitive prostate cancer (HSPC) into chemoresistant prostate cancer (CRPC), and may serve as a potential therapeutic target in CRPC.

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