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验证数据展示

  • WB analysis using 26707-1-AP

    WB analysis using 26707-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26707-1-AP (USP54 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 26707-1-AP

    IHC staining of mouse liver using 26707-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 26707-1-AP (USP54 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

USP54 Polyclonal antibody

USP54 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26707-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

C10orf29, Inactive ubiquitin carboxyl-terminal hydrolase 54, Inactive ubiquitin-specific peptidase 54

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26707-1-AP targets USP54 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 USP54 fusion protein Ag25080 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 54
别名 C10orf29, Inactive ubiquitin carboxyl-terminal hydrolase 54, Inactive ubiquitin-specific peptidase 54
观测分子量180 kDa
GenBank蛋白编号BC130633
基因名称 USP54
Gene ID (NCBI) 159195
RRIDAB_3669564
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ70EL1
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP54 is a ubiquitin-specific protease in the deubiquitinating enzyme (DUB) family that promotes the malignant progression of many cancers. As a deubiquitinating enzyme, USP54 maintains the stability of PLK4 protein through deubiquitination, which promotes centrosome amplification and GC progression. USP54 is also highly expressed in colorectal cancers, and is associated with proliferation, invasion and migration of colorectal cancer. In addition, USP54 plays a key role in the transformation of hormone-sensitive prostate cancer (HSPC) into chemoresistant prostate cancer (CRPC), and may serve as a potential therapeutic target in CRPC.

实验方案

Product Specific Protocols
WB protocol for USP54 antibody 26707-1-APDownload protocol
IHC protocol for USP54 antibody 26707-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols