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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated USP5 Monoclonal antibody

USP5 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3F12C10

Cat No : CL488-66213

Print datasheet

Synonyms

Deubiquitinating enzyme 5, Isopeptidase T, ISOT, Ubiquitin thiolesterase 5, USP5

验证数据展示

  • IF Staining of A549 using CL488-66213

    IF Staining of A549 using CL488-66213

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite® Plus 488 USP5 antibody (CL488-66213, Clone: 3F12C10 ) at dilution of 1:400.

  • FC experiment of A549 using CL488-66213

    FC experiment of A549 using CL488-66213

    1x10^6 A549 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488-conjugated USP5 Monoclonal antibody (CL488-66213, Clone:3F12C10)(red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inA549 cells
Positive FC detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 66213-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66213 targets USP5 in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen USP5 fusion protein Ag7575 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 5 (isopeptidase T)
Synonyms Deubiquitinating enzyme 5, Isopeptidase T, ISOT, Ubiquitin thiolesterase 5, USP5
Calculated Molecular Weight 96 kDa
Observed Molecular Weight 95-105 kDa
GenBank Accession NumberBC005139
Gene Symbol USP5
Gene ID (NCBI) 8078
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP45974
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP5, also named as ISOT, belongs to the peptidase C19 family. Knock-down of USP5 causes the accumulation of TP53/p53 and an increase in TP53/p53 transcriptional activity because the unanchored polyubiquitin that accumulates is able to compete with ubiquitinated TP53/p53 but not with MDM2 for proteasomal recognition. USP5 is a potential target for p53 activating therapeutic agents for the treatment of cancer(PMID: 19098288). It is a novel proteasome associated protein(PMID: 19182904). USP5 cleaves linear and branched multiubiquitin polymers with a marked preference for branched polymers.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 USP5 antibody CL488-66213Download protocol
FC protocol for CL Plus 488 USP5 antibody CL488-66213Download protocol
Standard Protocols
Click here to view our Standard Protocols