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  • KD/KO Validated

USP46 Polyclonal antibody

USP46 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB,ELISA and More (3)

Conjugate

Unconjugated

Cat No : 13502-1-AP

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Synonyms

Deubiquitinating enzyme 46, Ubiquitin thiolesterase 46, USP46



经过测试的应用

Positive WB detected inhuman kidney tissue, mouse pancreas tissue
Positive IHC detected inhuman kidney tissue, human pancreas tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13502-1-AP targets USP46 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB,ELISA
Cited ApplicationsWB, IP, IF, IHC, CoIP
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen USP46 fusion protein Ag4330 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ubiquitin specific peptidase 46
Synonyms Deubiquitinating enzyme 46, Ubiquitin thiolesterase 46, USP46
Calculated Molecular Weight 366 aa, 42 kDa
Observed Molecular Weight 38-42 kDa
GenBank Accession NumberBC037574
Gene Symbol USP46
Gene ID (NCBI) 64854
RRIDAB_10642951
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP62068
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for USP46 antibody 13502-1-APDownload protocol
IHC protocol for USP46 antibody 13502-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IP

Nat Commun

The USP46 complex deubiquitylates LRP6 to promote Wnt/β-catenin signaling

Authors - Victoria H Ng
mouseWB

J Exp Med

USP1-UAF1 deubiquitinase complex stabilizes TBK1 and enhances antiviral responses.

Authors - Zhongxia Yu
  • KD Validated
humanWB,IHC,CoIP,IF

Exp Cell Res

Ubiquitin-specific peptidase 46 promotes tumor metastasis through stabilizing ENO1 in human esophageal squamous cell carcinoma.

Authors - Maoqing Tian
  • KD Validated
humanWB

Cell Biol Int

Ubiquitin specific protease 46 potentiates triple negative breast cancer development by stabilizing PGAM1-mediated glycolysis

Authors - Longzhu Ke
  • KD Validated