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验证数据展示

  • WB analysis using 24559-1-AP (same clone as 24559-1-PBS)

    WB analysis using 24559-1-AP (same clone as 24559-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 24559-1-AP (USP35 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 24559-1-AP (same clone as 24559-1-PBS)

    IHC staining of human colon cancer using 24559-1-AP (same clone as 24559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 24559-1-AP (same clone as 24559-1-PBS)

    IHC staining of human colon cancer using 24559-1-AP (same clone as 24559-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 24559-1-AP (USP35 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24559-1-PBS in a different storage buffer formulation.

USP35 Polyclonal antibody, PBS Only

USP35 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 24559-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, Indirect ELISA

别名

EC:3.4.19.12, KIAA1372, Ubiquitin carboxyl-terminal hydrolase 35, Ubiquitin-specific-processing protease 35, USP34

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

24559-1-PBS targets USP35 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag20002

Product name: Recombinant human USP35 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-342 aa of BC131489

Sequence: MPGLRRRASWRVSIPGSWPSQTRARLVSSTWATHAMSTASFRPYSWRLSSFRHCVLRLTENNSQPLMTKLQWLFGFLEHSQRPAISPENFLSASWTPWFSPGTQQDCSEYLKYLLDRLHEEEKTGTRICQKLKQSSSPSPPEEPPAPSSTSVEKMFGGKIVTRICCLCCLNVSSREEAFTDLSLAFPPPERCRRRRLGSVMRPTEDITARELPPPTSAQGPGRVGPRRQRKHCITEDTPPTSLYIEGLDSKEAGGQSSQEERIEREEEGKEERTEKEEVGEEEESTRGEGEREKEEEVEEEEEKVEKETEKEAEQEKEEDSLGAGTHPDAAIPSGERTCGSE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 35
别名 EC:3.4.19.12, KIAA1372, Ubiquitin carboxyl-terminal hydrolase 35, Ubiquitin-specific-processing protease 35, USP34
计算分子量 1018 aa, 113 kDa
观测分子量 84 kDa
GenBank蛋白编号BC131489
基因名称 USP35
Gene ID (NCBI) 57558
RRIDAB_2879608
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P2H5
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

USP35 is a member of the peptidase C19 family. USP35 is involved in cell cycle regulation, cell fate, and cancer development. USP35 regulates cell mitosis by deubiquitinating Aurora B and prevents its degradation from APC/CDH. USP35 can also regulate MFN2 abundance and PARK2-mediated mitophagy. USP35 has 2 isoforms with the molecular mass of 84 and 113 kDa (PMID: 34618999).

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