验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells, HeLa cells, RAW 264.7 cells |
| Positive IHC detected in | human colon cancer tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
24559-1-AP targets USP35 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, CoIP |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | USP35 fusion protein Ag20002 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 35 |
| 别名 | USP34, Ubiquitin-specific-processing protease 35, Ubiquitin carboxyl-terminal hydrolase 35, KIAA1372, EC:3.4.19.12 |
| 计算分子量 | 1018 aa, 113 kDa |
| 观测分子量 | 84 kDa |
| GenBank蛋白编号 | BC131489 |
| 基因名称 | USP35 |
| Gene ID (NCBI) | 57558 |
| RRID | AB_2879608 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9P2H5 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP35 is a member of the peptidase C19 family. USP35 is involved in cell cycle regulation, cell fate, and cancer development. USP35 regulates cell mitosis by deubiquitinating Aurora B and prevents its degradation from APC/CDH. USP35 can also regulate MFN2 abundance and PARK2-mediated mitophagy. USP35 has 2 isoforms with the molecular mass of 84 and 113 kDa (PMID: 34618999).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for USP35 antibody 24559-1-AP | Download protocol |
| IHC protocol for USP35 antibody 24559-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Commun Biol Overexpression of USP35 enhances the protective effect of hUC-MSCs and their extracellular vesicles in oxygen-glucose deprivation/reperfusion-induced SH-SY5Y cells via stabilizing FUNDC1
| ||
Am J Physiol Cell Physiol USP35 promotes breast cancer progression by regulating PFK-1 ubiquitination to mediate glycolysis | ||
Cell Death Discov Deubiquitinase USP35 stabilizes BRPF1 to activate mevalonate (MVA) metabolism during prostate tumorigenesis | ||
Oncogenesis USP35 promotes cell proliferation and chemotherapeutic resistance through stabilizing FUCA1 in colorectal cancer | ||
Biochem Biophys Res Commun Regulation of survivin protein stability by USP35 is evolutionarily conserved |
