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验证数据展示

  • WB analysis using 15402-1-AP

    WB analysis using 15402-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15402-1-AP (USP30 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 15402-1-AP

    IHC staining of human liver cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human liver cancer using 15402-1-AP

    IHC staining of human liver cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human colon cancer using 15402-1-AP

    IHC staining of human colon cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 15402-1-AP

    IHC staining of human colon cancer using 15402-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15402-1-AP (USP30 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of A549 using 15402-1-AP

    IF Staining of A549 using 15402-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using USP30 antibody (15402-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • IF Staining of hela using 15402-1-AP

    IF Staining of hela using 15402-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using USP30 antibody (15402-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • IF Staining of HepG2 using 15402-1-AP

    IF Staining of HepG2 using 15402-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using USP30 antibody (15402-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

USP30 Polyclonal antibody

USP30 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15402-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA and More (1)

别名

Ubiquitin-specific-processing protease 30, Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inDaudi cells, HepG2 cells, hTERT-RPE1 cells, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman colon cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, A549 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15402-1-AP targets USP30 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 USP30 fusion protein Ag7620 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 30
别名 Ubiquitin-specific-processing protease 30, Ubiquitin thiolesterase 30, Ubiquitin thioesterase 30, Ubiquitin carboxyl-terminal hydrolase 30, EC:3.4.19.12
计算分子量58.5 kDa
观测分子量 59-67 kDa
GenBank蛋白编号BC004868
基因名称 USP30
Gene ID (NCBI) 84749
RRIDAB_3085460
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ70CQ3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP30, a mitochondrion-localized deubiquitylase, regulates mitophagy. Mitochondrial damage stimulates parkin to assemble Lys 6, Lys 11 and Lys 63 chains on mitochondria, and that USP30 is a ubiquitin-specific deubiquitylase with a strong preference for cleaving Lys 6- and Lys 11-linked multimers. USP30 can be ubiquitinated by parkin (PARK2) at Lys-235 and Lys-289. USP30 inhibition is potentially beneficial for treating Parkinson disease by promoting mitochondrial clearance and quality control. USP30 was detected 67 kDa by ubiquitination (PMID: 34704599).

实验方案

Product Specific Protocols
WB protocol for USP30 antibody 15402-1-APDownload protocol
IHC protocol for USP30 antibody 15402-1-APDownload protocol
IF protocol for USP30 antibody 15402-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Biochem Biophys Res Commun

USP30 impairs mitochondrial quality control and aggravates oxidative damage after traumatic brain injury

Authors - Yang Wu
View Article
IF

Nature

Immune evasion through mitochondrial transfer in the tumour microenvironment

Authors - Hideki Ikeda
View Article