验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, HeLa cells, HepG2 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human testis tissue, human brain tissue, human spleen tissue, human lung tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
17491-1-AP targets USP20 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, IP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag11590 Product name: Recombinant human USP20 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC039593 Sequence: MGDSRDLCPHLDSIGEVTKEDLLLKSKGTCQSCGVTGPNLWACLQVACPYVGCGESFADHSTIHAQAKKHNLTVNLTTFRLWCYACEKEVFLEQRLAAPLLGSSSKFSEQDSPPPSHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLVSEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPVVATVALTEARDSDSSDTDEKREGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSQAETELLIPDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVD 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 20 |
| 别名 | Deubiquitinating enzyme 20, EC:3.4.19.12, hVDU2, KIAA1003, Ubiquitin carboxyl-terminal hydrolase 20 |
| 计算分子量 | 914 aa, 102 kDa |
| 观测分子量 | 110-120 kDa |
| GenBank蛋白编号 | BC039593 |
| 基因名称 | USP20 |
| Gene ID (NCBI) | 10868 |
| RRID | AB_2212570 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y2K6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP20(ubiquitin carboxyl-terminal hydrolase 20) is also named as KIAA1003, LSFR3A, VDU2 and belongs to the peptidase C19 family and the USP20/USP33 subfamily. It can specifically deubiquitinate and stabilize HIF1A and, therefore, increase expression of HIF-1alpha targeted genes, such as vascular endothelial growth factor (VEGF). It also plays a central role in ADRB2 recycling and resensitization after prolonged agonist stimulation by constitutively binding ADRB2, mediating deubiquitination of ADRB2 and inhibiting lysosomal trafficking of ADRB2.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for USP20 antibody 17491-1-AP | Download protocol |
| IP protocol for USP20 antibody 17491-1-AP | Download protocol |
| WB protocol for USP20 antibody 17491-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Genes Dev Deubiquitinase USP20 promotes breast cancer metastasis by stabilizing SNAI2.
| ||
J Cell Biol Deubiquitinases USP20/33 promote the biogenesis of tail-anchored membrane proteins. | ||
Cell Death Differ USP20 positively regulates tumorigenesis and chemoresistance through β-catenin stabilization.
| ||
Diabetes Hepatokine ERAP1 Disturbs Skeletal Muscle Insulin Sensitivity via Inhibiting USP33-Mediated ADRB2 Deubiquitination. | ||
Front Oncol USP20 is a predictor of poor prognosis in colorectal cancer and associated with lymph node metastasis, immune infiltration and chemotherapy resistance
| ||
Mol Ther Oncolytics USP18 and USP20 restrict oHSV-1 replication in resistant human oral squamous carcinoma cell line SCC9 and affect the viability of SCC9 cells.
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