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CoraLite® Plus 488-conjugated USP14 Monoclonal antibody

USP14 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

3B12G3

Cat No : CL488-67746

Print datasheet

Synonyms

Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14

验证数据展示

  • IF Staining of HepG2 using CL488-67746

    IF Staining of HepG2 using CL488-67746

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 USP14 antibody (CL488-67746, Clone: 3B12G3 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67746-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67746 targets USP14 in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, Mouse, Rat
Immunogen USP14 fusion protein Ag6312 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase)
Synonyms Deubiquitinating enzyme 14, TGT, Ubiquitin thiolesterase 14, USP14
Calculated Molecular Weight 54 kDa
Observed Molecular Weight60 kDa
GenBank Accession NumberBC003556
Gene Symbol USP14
Gene ID (NCBI) 9097
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP54578
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP14(Ubiquitin carboxyl-terminal hydrolase 14) is also named as TGT and belongs to the peptidase C19 family. Mammalian USP14 is unique among known UBP enzymes in that it is activated catalytically upon specific association with the 26S proteasome. USP14 inhibition accelerated the degradation of oxidized proteins and enhanced resistance to oxidative stress.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 USP14 antibody CL488-67746Download protocol
Standard Protocols
Click here to view our Standard Protocols