验证数据展示
经过测试的应用
| Positive IF/ICC detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy USP11 Ready-To-Use IHC Kit. USP11 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67805-1-Ig
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-67805 targets USP11 in IF/ICC applications and shows reactivity with Human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | Human |
| 免疫原 | USP11 fusion protein Ag19058 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | ubiquitin specific peptidase 11 |
| 别名 | Deubiquitinating enzyme 11, Ubiquitin thiolesterase 11, UHX1, USP11 |
| 计算分子量 | 963 aa, 110 kDa |
| 观测分子量 | 110 kDa |
| GenBank蛋白编号 | BC000350 |
| 基因名称 | USP11 |
| Gene ID (NCBI) | 8237 |
| RRID | AB_2923925 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P51784 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
USP11(Ubiquitin carboxyl-terminal hydrolase 11), also named as UHX1, is a deubiquitinating enzyme controling many intracellular processes, including cell cycle progression, transcriptional activation, and signal transduction. There are four bands of 120 kDa, 55 kDa, 50 kDa and 44 kDa to be detected in HeLa extract by western blot and the smaller proteins may be degradation products, some other spliced products could possibly correspond to the smaller proteins and possess functions(PMID:12084015). This protein belongs to the peptidase C19 family.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 USP11 antibody CL594-67805 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |