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CoraLite® Plus 488-conjugated USP10 Monoclonal antibody

USP10 Monoclonal Antibody for FC (Intra), IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

FC (Intra), IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1B10C9

Cat No : CL488-67917

Print datasheet

Synonyms

Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10

验证数据展示

  • IF Staining of HEK-293 using CL488-67917

    IF Staining of HEK-293 using CL488-67917

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 USP10 antibody (CL488-67917, Clone: 1B10C9 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67917

    FC experiment of HeLa using CL488-67917

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human USP10 (CL488-67917, Clone:1B10C9) (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF detected inHEK-293 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67917-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67917 targets USP10 in IF, FC (Intra) applications and shows reactivity with Human, mouse, rat samples.

Tested Applications FC (Intra), IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen USP10 fusion protein Ag7233 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name ubiquitin specific peptidase 10
Synonyms Deubiquitinating enzyme 10, KIAA0190, Ubiquitin thiolesterase 10, UBPO, USP10
Calculated Molecular Weight 87 kDa, 92 kDa
Observed Molecular Weight100-110 kDa
GenBank Accession NumberBC000263
Gene Symbol USP10
Gene ID (NCBI) 9100
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14694
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP10, a cytoplasmic ubiquitin-specific protease, deubiquitinates p53, reversing Mdm2-induced p53 nuclear export and degradation. After DNA damage, USP10 is stabilized, and a fraction of USP10 translocates to the nucleus to activate p53. The translocation and stabilization of USP10 is regulated by ATM -mediated phosphorylation of USP10 at Thr42 and Ser337. (PMID: 20096447, PMID: 26876938)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 USP10 antibody CL488-67917Download protocol
FC protocol for CL Plus 488 USP10 antibody CL488-67917Download protocol
Standard Protocols
Click here to view our Standard Protocols