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验证数据展示

  • IF Staining of HEK-293 using CL488-67917

    IF Staining of HEK-293 using CL488-67917

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CoraLite® Plus 488 USP10 antibody (CL488-67917, Clone: 1B10C9 ) at dilution of 1:200.

  • FC experiment of HeLa using CL488-67917

    FC experiment of HeLa using CL488-67917

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human USP10 (CL488-67917, Clone:1B10C9) (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated USP10 Monoclonal antibody

USP10 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67917

产品说明书

CloneNo. 1B10C9

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Ubiquitin-specific-processing protease 10, Ubiquitin carboxyl-terminal hydrolase 10, KIAA0190, EC:3.4.19.12, Deubiquitinating enzyme 10

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 67917-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67917 targets USP10 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7233

Product name: Recombinant human USP10 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 444-798 aa of BC000263

Sequence: KFIPLYSKVQRPCTSTPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEPTYIYRLLTVNKSSLSEKGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPKNHSVNEEEQEEQGEGSEDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDIQSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKTGGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTDVFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 ubiquitin specific peptidase 10
别名 Ubiquitin-specific-processing protease 10, Ubiquitin carboxyl-terminal hydrolase 10, KIAA0190, EC:3.4.19.12, Deubiquitinating enzyme 10
计算分子量 87 kDa, 92 kDa
观测分子量100-110 kDa
GenBank蛋白编号BC000263
基因名称 USP10
Gene ID (NCBI) 9100
RRIDAB_2923825
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ14694
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

USP10, a cytoplasmic ubiquitin-specific protease, deubiquitinates p53, reversing Mdm2-induced p53 nuclear export and degradation. After DNA damage, USP10 is stabilized, and a fraction of USP10 translocates to the nucleus to activate p53. The translocation and stabilization of USP10 is regulated by ATM -mediated phosphorylation of USP10 at Thr42 and Ser337. (PMID: 20096447, PMID: 26876938)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 USP10 antibody CL488-67917Download protocol
Standard Protocols
Click here to view our Standard Protocols
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